Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

描述(由申请者提供)：俄克拉荷马州内森休克中心将专注于新发展的老年科学领域，并为全国各地的研究人员提供独特和创新的分析方法，整个研究老龄化和老年科学的研究社区都可以使用这些方法。俄克拉荷马州内森休克中心的5个核心是：计划增强核心：此外，为了监督休克中心的所有活动，计划增强核心将管理两个新的计划。俄克拉荷马州老年科学联盟的发展将汇集来自俄克拉荷马州五大研究机构的对老龄化和老年科学感兴趣的研究人员。此外，通过发现生物信息学组件，核心将为老龄化研究人员提供独特的生物信息学工具和方法，以发现他们的数据和与年龄相关的现象之间的新联系。研究开发核心：该核心是对内森冲击中心类似核心的传统结构的创新。俄克拉荷马州内森休克中心的研究开发核心旨在为整个科学界提供前所未有的服务，而不仅仅是主办机构的研究人员。多重蛋白质量化核心：这一核心将为研究人员提供一种新的质谱学方法，用于测量来自任何组织/细胞的蛋白质的丰度，这些组织/细胞来自任何已对其基因组进行排序的有机体。Core可以在一次运行中对25到30个蛋白质的面板进行量化，从而使研究人员能够询问衰老和老年科学中的重要途径。通过开发线虫、果蝇、酵母以及特别长寿动物的蛋白质面板，该核心将首次为研究人员提供一种在这些动物模型中测量蛋白质丰度的方法，这些蛋白质对衰老很重要。靶向DNA甲基化和线粒体异质性核心：利用目前仅在该核心中提供的独特的引物组的新的下一代测序，研究人员将有可能研究基因组范围或特定基因中的DNA甲基化。此外，核心开发的方法将使研究人员能够以高通量的方式对线粒体基因组异质性(变异/突变和缺失)和从非常小的样本中复制数量进行全面分析。综合氧化还原生物学核心：这一核心将为研究人员提供对细胞氧化应激状态的全面最先进的评估，例如，氧化损伤和氧化还原对的测量，这需要主要的仪器和专业知识来获得准确和可重复的数据。在过去，这个核心的领导人经历了全国各地调查人员对这些分析的高需求。

DESCRIPTION (provided by applicant): The Oklahoma Nathan Shock Center will focus on the newly-developing field of Geroscience and on providing investigators across the nation with unique and innovative assays that are accessible to the entire research community studying aging and Geroscience. The 5 Cores in the Oklahoma Nathan Shock Center are: Program Enhancement Core: In addition, to overseeing all the activities of the Shock Center, the Program Enhancement Core will administer two novel programs. The development of the Oklahoma Geroscience Consortium will bring together investigators interested in aging and Geroscience from the five major research institutions in Oklahoma. In addition, through a Discovery Bioinformatics Component, the Core will provide investigators in aging with unique bioinformatics tools and methods to discover new connections between their data and age-related phenomenon. Research Development Core: This Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers. The Research Development Core of the Oklahoma Nathan Shock Center is designed to provide unprecedented service to the scientific community at large, not just investigators at the host institution. Multiplexing Protein Quantification Core: This Core will provide investigators with a novel mass spectrometry method for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of 25 to 30 proteins in a single run, thereby allowing investigators to interrogate pathways of importance in aging and Geroscience. By developing panels of proteins for C elegans, Drosophila, and yeast, as well as exceptionally long-lived animals, this Core will provide investigators, for the first-time, a method for measurin protein abundance in these animal models, which are important to aging. Targeted DNA Methylation & Mitochondrial Heteroplasmy Core: Using novel next-generation sequencing with unique primer sets, which are currently available only in this Core, it will be possible for investigators to study DNA methylation either genome-wide or in specific genes. In addition, the Core has developed methods that will allow investigators to conduct a comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number from very small samples in a high throughput manner. Integrative Redox Biology Core: This Core will provide investigators with a comprehensive state-of-the-art assessment of the oxidative stress status of a cell, e.g., measures of oxidative damage and redox couples, which require major instrumentation and expertise to obtain accurate and reproducible data. In the past, the Leaders of this Core have experienced a high demand for these assays from investigators across the country.