Operetta CLS High-content Imaging System

项目总结/摘要 高内容筛选采用基于成像的平台，该平台结合了 高通量自动化的效率和速度以及光学显微镜的力量， 特别是荧光显微镜，以同时捕获功能和 来自大量细胞或生物体样品的形态测量信息 干扰后，如药物治疗或基因改变。高含量 分析允许对药物进行更全面的定量评估， 基因效应比基于群体的方法，因为多个动力学和空间 可以在各个单元中同时询问读出。本申请寻求 填补了对高内容筛选的强烈需求和有限的 弗吉尼亚大学目前可以提供的能力。拟议的文书是 PerkinElmer Operetta CLS是一款能够实现亮场的高容量成像系统， 相衬和宽视场和旋转盘共焦荧光成像。的 收购将立即有利于广泛的生物医学研究项目 包括流感、埃博拉、心血管疾病等传染病， 肥胖、癌症、外周和中枢神经变性。有了轻歌剧CLS， 可以同时捕获多个参数 候选人对已知的目标，以及发现未知的新目标， 对生物学问题的探索额外的好处是无偏差的图像采集 强大的数据分析。轻歌剧CLS定位于关键的新研究 工具，以促进我们广泛的基础或临床研究计划，这是 试图了解和寻找人类疾病的治疗方法。

Project Summary/Abstract High-content screening employs an imaging-based platform, which combines the efficacy and speed of high-throughput automation and the power of light microscopy, especially fluorescence microscopy, to simultaneously capture both functional and morphometric information from a large number of samples of cells or organisms following perturbation, such as drug treatment or genetic alteration. High content analysis permits a much more comprehensive quantitative assessment of drug and gene effects than population-based methods, because multiple kinetic and spatial readouts can be simultaneously interrogated in individual cells. This application seeks to fill the gap between a strong demand for high-content screening and the limited capability the University of Virginia can currently offer. The proposed instrument is a PerkinElmer Operetta CLS, a high-content imaging system capable of bright-field, phase-contrast, and wide-field and spinning-disc confocal fluorescence imaging. The acquisition will immediately benefit a wide range of biomedical research projects covering infectious diseases such as influenza, Ebola, cardiovascular disease, obesity, cancer, peripheral and central neurodegeneration. With the Operetta CLS, multiple parameters can be captured simultaneously for evaluation of tool or drug candidates against known targets as well as for uncovering unknown new targets in the exploration of a biological problem. Added benefit is unbiased image acquisition and powerful data analysis. The Operetta CLS is positioned to critical new research tool to facilitate our extensive basic or clinical research programs, which are attempting to understand and find treatments for human diseases.