Injection of autologous volar fibroblasts to the stump site to allow pressure adaptation and enhanced prosthetic use in amputees

项目摘要摘要 这项拟议研究的长期目标是使用细胞疗法来推进再生医学。 特别是，我们希望帮助失去肢体的截肢者。虽然已经对以下方面进行了重大改进 假体，残端界面的皮肤一侧没有进化到能够承受重量。树桩不会 例如，正常情况下有脚底的压力适应能力。目前的赠款建议 从现有的手掌或脚掌中活检、纯化和扩增成纤维细胞，并将它们注射到 截肢者改善他们的假肢使用情况。我们还建议研究细胞疗法背后的基础生物学，以 了解注射的细胞如何改变原生细胞，进而了解这些原生细胞如何影响注射的 细胞。这笔赠款的结果有望带来一种新的治疗方法，不仅帮助截肢者，而且有可能 预防压疮，为未来的细胞疗法提供平台。

Project Summary Abstract The long term objective of the proposed research is to use cellular therapy to advance regenerative medicine. In particular, we wish to help amputees who have lost a limb. While major improvements have been made to prosthetics, the skin side of the interface at the stump site was not evolved to bear weight. The stump does not normally have the pressure adaptive abilities of the soles of the foot for example. The present grant proposes to biopsy, purify and expand fibroblasts from an existing palm or sole and inject them into the stump site of an amputee to improve their use of prosthetics. We also propose to study the basic biology behind cell therapy to understand how the injected cells change the native cells, and in turn how those native cells affect the injected cells. The results of this grant will hopefully lead to a new therapy to help not only amputees, but potentially prevent pressure ulcers and provide a platform for future cell therapies.