Core C - Bioanalytical Resource Core

项目摘要 生物分析核心（核心C）服务于奥布莱恩中心社区，无论是在UAB和UCSD， 通过提供最先进的生物能量学、氧化应激分析和代谢产物分析， 和生物标志物分析支持急性肾损伤（阿基）研究。核心C提供了一个全面的 资源，包括氧化应激生物分析的优化方案和技术， 细胞生物能量学、阿基生物标志物、翻译后修饰和小分子 生物化学服务内容包括咨询、实验设计培训、回收合适的样品 用于分析和测定性能。提供的服务包括独特的技术，以确定线粒体 生物能量学、基于LC-质谱的肌酐、F2-异前列烷和柠檬酸循环测定 中间体的通过核心C提供的新服务包括肾脏成像质谱 组织，用于临床靶向和非靶向代谢组学的最先进的nanoLC-MS方法 样品和来自阿基的动物模型。 生物分析中心继续为研究者提供独特的服务组合 奥布莱恩中心自2013年以来，Core C已为187个国家提供了超过53，000次服务/检测。 调查涉及226个项目。在187个核心用户中，155个是非核心调查员。核心C 为17个试点项目、可行性和催化剂赠款获得者提供了支持。这些共同努力 在86篇同行评审的出版物中得到认可。Core C的生产力基准测试表明， >300%用于调查人员（特别是非核心调查人员）、支助的项目以及出版物 与上一个融资周期相比。核心小组参与调查人员的教育和培训， 实践经验和科学交流。一个经常性的特点是每年举办一次为期4.5天的培训讲习班 在代谢组学中。生物分析核心与奥布莱恩中心领导层合作， 催化剂，以支持研究人员推进肾脏相关的研究。

Project Summary The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the Extended Research bBase by providing state-of-the-art bioenergetics, oxidative stress analysis, and metabolite and biomarker analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. Services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. New services being offered through Core C include imaging mass spectrometry for kidney tissues, state-of-the-art nanoLC-MS methods for both targeted and untargeted metabolomics in clinical samples and from animal models of AKI. The Bioanalytical Core has continued to provide a unique portfolio of services for the investigator base of the O'Brien Center. Since 2013, Core C has performed more than 53,000 services/assays for 187 investigators involving 226 projects. Of the 187 core users, 155 were non-core investigators. Core C also provided support for 17 pilot and feasibility and catalyst grant awardees. These combined efforts have been recognized in 86 peer-reviewed publications. Productivity benchmarks for Core C demonstrate an increase of >300% for investigators (especially non-core investigators), supported projects as well as publications compared to the prior funding cycle. The Core participates in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature is an annual 4.5-day workshop for training in metabolomics. The Bioanalytical Core, in concert with the O'Brien Center leadership, provides a strong catalyst to support investigators to advance kidney-related research.