Male accessory gland proteins of the dengue vector mosquito Aedes aegypti: potent

描述(申请人提供)：在这项研究中，我们将使用一种新的基因组学工具和实地研究相结合的方法来研究登革热媒介蚊子埃及伊蚊雄性生殖蛋白(附腺蛋白的ACP)的功能及其作为遗传控制策略目标的可能性。雄性ACP首先在果蝇身上被发现，在那里它们被证明对雌性的生殖生物学有戏剧性的影响。从雄性果蝇中发现的单个ACPs影响雌性产卵和产卵、精子储存、交配和取食行为。早期对Ae.埃及伊蚊证明了一种名为“麦特龙”的雄性附腺物质对雌性行为和生理的影响。雌雄配子或副腺液注入雌性Ae。埃及伊蚊对雌性性不稳定、取血行为、血液消化、飞行、卵巢发育和产卵都有影响。这项早期工作和我们自己的初步数据表明，男性ACP将在Ae中发挥重要作用。埃及伊蚊，因此必须对它们进行详细调查并确定它们的功能。我们将检验假设，即Ae。埃及伊蚊雄性在交配过程中将ACPs传递给雌性蚊子，这些蛋白质对雌性蚊子的生物学和生殖适合性有重大影响。最终，这些ACP可能会影响推动媒介传播疾病动态的各种女性特征。公共卫生相关性在本研究中，我们将鉴定登革热媒介蚊子埃及伊蚊的雄性生殖蛋白。我们将通过实验室和现场研究的新组合，研究这些蛋白质对雌性媒介生物学的影响，以及它们作为基因控制策略目标的潜力。

DESCRIPTION (provided by applicant): In this study we will use a novel combination of genomics tools with field studies to address the function of male reproductive proteins (Acps for Accessory gland proteins) of the dengue vector mosquito, Aedes aegypti, and their potential as targets for genetic control strategies. Male Acps were first identified in Drosophila melanogaster, where they have been shown to have dramatic effects on the reproductive biology of the female. Individual Acps identified from male Drosophila affect female egg production and egg laying, sperm storage, mating and feeding behaviors. Earlier studies with Ae. aegypti demonstrated an effect of "matrone", a partially purified male accessory gland substance, on female behavior and physiology. Matrone or accessory gland fluid injected into female Ae. aegypti has affected female sexual refractoriness, blood feeding behavior, blood digestion, flight, ovarian development, and oviposition. This early work and our own preliminary data indicate that male Acps will be important in Ae. aegypti and makes it imperative to investigate them in detail and determine their function. We will test the hypothesis that Ae. aegypti males transfer Acps to females during mating, and that these proteins have a significant impact on the female mosquito biology and reproductive fitness. Ultimately, these Acps may influence a variety of female traits that drive vector-borne disease dynamics. PUBLIC HEALTH RELEVANCE In this study we will identify male reproductive proteins of the dengue vector mosquito, Aedes aegypti. We will investigate the impact of these proteins on female vector biology and their potential as targets for genetic control strategies though a novel combination of laboratory and field studies.