Cell Morphology and Pathology Core

细胞形态学和病理学核心： 细胞形态学和病理学核心的目标是为基因治疗中心的研究人员和对开发囊性纤维化（CF）和其他遗传疾病的基因转移方法感兴趣的新研究人员提供服务和指导。我们致力于为研究人员在人类疾病的新的大型和小型动物模型中使用基因转移工具解释疾病病理提供专家帮助。为了促进这些目标的实现，该中心提供：1）劳动密集型技术的技术援助，如从组织或细胞培养物制备切片，2）随时获得设备，3）通过集中设备和设施的经济效益，以及4）专业形态学技术和图像分析方法的咨询和指导。这些目标是通过核心主任的监督实现的，核心主任是一位成熟的CF科学家，在基因治疗研究中有用的各种显微镜技术方面具有丰富的经验。 细胞形态学和病理学核心的具体目标是： 1)为中心研究者提供适当的组织固定、处理、冷冻保存、石蜡或塑料包埋、切片和常规染色方法。 2)对于需要检测报告蛋白的研究者，提供染色技术方面的帮助，例如β-半乳糖苷酶活性的检测和细胞特异性定位。 3)利用组织化学和荧光技术为需要检测特定蛋白质的细胞和组织的研究者提供蛋白质抗体标记的帮助。 4)对于需要电子显微镜（TEM或SEM）或共聚焦显微镜的研究者，提供组织固定、处理和显微照片、计算机分析和结果解释方面的帮助。 5)对于需要原位杂交的研究者，提供组织制备和探针检测的指导和帮助。 6)为疾病动物模型中显微镜到大体标本的检查和解释及其对基因疗法的反应提供专家兽医病理学支持。 7)协助研究者开发和使用与基因转移相关的新的和/或专门的形态学方法。

Cell Morphology and Pathology Core: The goals of the Cell Morphology and Pathology Core are to provide services and instruction to investigators of the Gene Therapy Center and to new investigators interested in developing gene transfer approaches to cystic fibrosis (CF) and other genetic diseases. We strive to provide expert assistance in the interpretation of disease pathology as investigators use gene transfer tools in new large and small animal models of human disease. To facilitate these goals the Core provides: 1) technical assistance for labor- intensive techniques such as the preparation of sections from tissue or cell cultures, 2) ready access to equipment, 3) economic benefits through centralization of equipment and facilities, and 4) consultation and instruction in specialized morphologic techniques and methods of image analysis. These goals are met through oversight by the Core Director, an established CF scientist with experience in a variety of microscopy techniques that are useful in gene therapy research. The specific aims of the Cell Morphology and Pathology Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators. 2) For investigators who require detection of reporter proteins, to provide assistance with staining techniques, such as detection and cell-specific localization of (J-galactosidase activity. 3) To provide assistance with antibody labeling of proteins using histochemical and fluorescence techniques to investigators who require cell and tissue detection of specific proteins. 4) For investigators who require electron microscopy (TEM or SEM) or confocal microscopy, to provide tissue fixation, processing, and assistance in photo micrography, computerized analysis and interpretation of results. 5) For investigators who require in situ hybridization, to provide instruction and assistance in tissue preparation and detection of the probe. 6) To provide expert veterinary pathology support for the examination and interpretation of microscopic to gross specimens in animal models of disease and their response to gene-based therapies. 7) To provide assistance to investigators in the development and use of new and/or specialized morphological methods relevant to gene transfer.