Acquisition of a Q Exactive HF Hybrid Quadrupole-Orbitrap

摘要 我们正在重新提交一份改进和更新的提案，要求提供资金，用于购买 Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS系统将在马萨诸塞州运行 光谱服务（MSS）的核心设施在化学系。MSS服务更多 来自12个部门和学院的UF校园90多个研究小组。30多 非UF学术机构和工业公司也利用MSS设施。我们 要求这种仪器，因为它的能力非常适合各种研究 项目包括大型和小型生物分子，未知物的表征和定量数据 在一个平台上。 Q Exactive Plus LC-MS/MS是一种串联质谱仪， 使用高分辨率/高质量精度Orbitrap质量分析仪进行前体选择。 完整的系统将包括可选的蛋白质模式，用于增强完整蛋白质的分析 和蛋白质复合物，UltiMate 3000 RSLCnano LC系统，Nanospray Flex离子源和 在毛细管流和纳米流之间改变系统所需的管路，BiosciomaTM 1.0，以及 Proteome Discoverer 2.1碱基和定量。 代谢组学、蛋白质组学和脂质组学领域现在被认为在以下方面发挥着关键作用： 系统生物学，并正在成为诊断测试，新药开发的重点 治疗，个性化医疗，以及对传染病，癌症和代谢的理解 紊乱拟议的Q Exactive HF Orbitrap系统将用于测量复杂混合物 蛋白质、肽、脂质、代谢物和其他需要超高分辨率串联的生物分子 用于检测、鉴定和定量那些复杂混合物的质谱。该数据 反过来，将改善本项目中与健康相关的目标的结果。 建议，例子包括：理解创伤性脑损伤;理解泛素- 蛋白酶体在治疗包括多发性骨髓瘤和套细胞在内的人类癌症中的应用 淋巴瘤;了解HIV-1蛋白酶耐药性;研究腺相关病毒（AAV） 作为基因治疗的递送载体或载体;开发生物传感器，生物分离方法， 通过结合适体和纳米材料进行靶向药物递送和癌细胞治疗;以及 开发和理解驱虫药的作用机制。

ABSTRACT We are resubmitting an improved and updated proposal requesting funds for the purchase of a Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS system to be operated in the Mass Spectrometry Services (MSS) core facility in the Department of Chemistry. The MSS serves more than 90 research groups on the UF campus from twelve departments and colleges. More than 30 non-UF academic institutions and industrial companies also utilize the MSS facility. We are requesting this instrument because its capabilities are well suited for diverse range of research projects including large- and small-biomolecules, characterization of unknowns, and quantitative data on a single platform. The Q Exactive Plus LC-MS/MS is a tandem mass spectrometer that combines a quadrupole mass analyzer for precursor selection with a high-resolution/high mass accuracy Orbitrap mass analyzer. The complete system will include the optional Protein Mode for enhanced analysis of intact proteins and protein complexes, an UltiMate 3000 RSLCnano LC system, a Nanospray Flex Ion Source and tubing required to change the system between capillary and nano flow, Biopharma Finder 1.0, and Proteome Discoverer 2.1 Base and Quantitation. The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders. The proposed Q Exactive HF Orbitrap system will be used for measuring complex mixtures of proteins, peptides, lipids, metabolites and other biomolecules requiring ultra-high resolution tandem mass spectrometry for detection, identification, and quantitation of those complex mixtures. This data will in turn improve the outcomes of the health related goals of the projects assembled in this proposal, examples including: understanding traumatic brain injury; understanding the ubiquitin- proteasome in the treatment of human cancers including multiple myeloma and mantle cell lymphoma; understanding drug resistance to HIV-1 protease; studying Adeno-associated virus (AAV) as a delivery vehicle or vector for gene therapy; developing biosensors, bioseparation methods, targeted drug delivery, and cancer cell treatment by combining aptamers and nanomaterials; and developing and understanding the mechanism of anthelmintics.