A National Glycoscience Resource - CCRC Service and Training

项目摘要 该提案的目标是创建一个国家资源在糖科学，将提供服务和手- 对所有类别的成分分析和详细的结构表征进行培训， 糖缀合物，包括糖蛋白、多糖和糖脂，以更大的科学价值， 社区格鲁吉亚大学的复合碳水化合物研究中心（CCRC） 两个NIH/NIGMS联邦资助的资源中心的所在地：国家生物医学糖组学中心 （5 P41 GM 103490），其中质谱和转录组分析的新技术已经被 开发用于研究干细胞和分化细胞类型的糖组学和糖蛋白质组学; 综合糖技术资源（5 P41 GM 103390），其中质谱、NMR、分子 建模、化学合成和基于细胞的测定已被用于研究 蛋白质-碳水化合物相互作用CCRC服务设施提供各种补充服务 利用两种研究资源开发的技术，这些技术现在已经成熟，完全可以翻译， 研究任何组织或细胞类型的糖组和糖蛋白组。NIGMS研究资源 最近已经日落，我们建议联合收割机从每个资源的“高需求”服务， 创建一个“国家糖科学资源”-CCRC服务和培训（GlyCore），使我们能够继续 提供并加强我们在糖科学、实践培训课程和研讨会方面的尖端服务， 使生物医学科学界能更容易地使用它们。GlyCore将提供一个全面的清单， 服务，包括分离和纯化，以及所有糖缀合物的NMR和MS表征， 被分为几个类别，并被整合在一起，以帮助解决糖科学中的特定问题。 为了更好地向外部调查人员提供我们的技术，我们还将继续提供四个良好的- 接受和完善的实践培训课程的糖科学。我们的经验表明， 我们的一对一培训形式在实现糖科学工具的接受方面非常有效。我们 拥有最先进的核磁共振和质谱仪器和训练有素的科学家， 在碳水化合物结构分析服务和培训方面的专业知识和业绩记录。我们全面贯彻 以及关于样本接收、样本处理、样本跟踪、存储、数据存档和 报告，为我们的用户提供统一质量的安全和分析输出。研究数量 受我们服务和培训活动影响的团体遍布全国， 在全球我们通过广泛的外联活动，建立了一个易于使用的“去”服务和培训设施 活动，包括我们在会议，研讨会和研讨会上的Glyco-booth， 与大学，科学非营利组织和行业的关系。

PROJECT SUMMARY The goal of this proposal is to create a National Resource in Glycoscience that will offer service and hands- on training for compositional analysis and detailed structural characterization of all classes of glycoconjugates, including glycoproteins, polysaccharides, and glycolipids, to the greater scientific community. The Complex Carbohydrate Research Center (CCRC) at the University of Georgia has been home to two NIH/NIGMS federally funded resource centers: the National Center for Biomedical Glycomics (5P41GM103490), where new technologies in mass spectrometry and transcriptome analysis have been developed to investigate glycomics and glycoproteomics of stem and differentiated cell types; and the Research Resource for Integrated Glycotechnology (5P41GM103390), in which mass spectrometry, NMR, molecular modeling, chemical synthesis and cell based assays have been used to investigate the molecular basis of protein-carbohydrate interactions. The CCRC service facility has offered a variety of complementary services using technologies developed by both research resources that are now mature and completely translatable to investigating the glycomes and glycoproteomes of any tissue or cell type. Since the NIGMS Research Resources have recently been sunsetted, we propose to combine the “high demand” services from each resource and create a “National Glycoscience Resource”-CCRC Service and Training (GlyCore) that will enable us to continue to offer and enhance our cutting edge services in glycoscience, hands-on training courses and workshops and to make them easily accessible to the biomedical science community. GlyCore will offer a comprehensive list of services, including isolation and purification, as well as NMR and MS characterization of all glycoconjugates that are grouped into several categories and are integrated together to help solve particular problems in glycoscience. In order to better make our technologies available to outside investigators, we will also continue to offer four well- received and well-established hands-on training courses in glycoscience. Our experience has demonstrated that the one-on-one format of our training is extremely effective in achieving acceptance of tools in glycoscience. We have a combination of state-of-the-art NMR and MS instruments and well-trained scientists with particular expertise and track record for service and training in carbohydrate structural analysis. We have fully implemented and well-developed protocols for sample receipt, sample handling, sample tracking, storage, data archiving, and reporting that provide our users with security and analytic outputs of uniform quality. The number of research groups that have been impacted by our service and training activities spans the whole country and extend globally. We have built an easily accessible “go-to” service and training facility through extensive outreach activities, including our Glyco-booth at meetings, seminars, and workshops at conferences, and building close relationships with universities, scientific non-profit organizations, and industries.