Gene Transfer Core, NIDCR-DIR

在2019财年，Gene Transfer Core成功提供了以下服务： 原核注射服务：注射12个转基因构建体以产生60个转基因株系。 基于CRISPR的基因编辑敲除服务：基于CRISPR的构建体产生KO和KI小鼠的平均效率分别为18%和17%。这项服务共产生了32个转基因小鼠品系。 冷冻保存和IVF服务：为7名研究者冷冻保存了28只小鼠模型的精子。来自所有小鼠模型的精子储存在两个单独的建筑物中。解冻42个精子管，进行149次IVF。根据研究者的要求，精子冷冻保存服务成功地重建了9只小鼠模型。 基因分型服务：对6名研究者的582份尾部活检标本进行纯化并用于PCR分析基因分型。

In FY19, Gene Transfer Core successfully provided following services: Pronuclear injection service: 12 transgenic constructs were injected to generate 60 transgenic lines. CRISPR-based gene editing knockout service:The average efficiency for generating KO and KI mice for CRISPR-based constructs was 18% and 17%, respectively. A total of 32 transgenic mouse lines were generated by this service. Cryopreservation and IVF services: Sperm from 28 mouse models were cryopreserved for 7 investigators. Sperm from all mouse models were stored in two separate buildings. 42 sperm straws were thawed for 149 IVF sessions. 9 mouse models were successfully re-established per investigators request by the sperm cryopreservation service. Genotyping service: 582 tail biopsies were purified and used for genotyping by PCR analysis for 6 investigators.