Shared Resource 05: Collaborative Functional Genomics

协同功能基因组学共享资源 项目摘要/摘要 了解癌症发生和发展的基本生物学机制是 这是找到更好的癌症预防和治疗方法的基础。功能丧失遗传 方法，如高通量RNA干扰(RNAi)和CRISPR/CAS9，以及功能增益 开放阅读框架(ORF)过表达和CRISPR激活(CRISPR-a)等方法提供了 识别新的癌症相关基因和途径的尖端战略。协作性功能 基因组学核心提供了一个单一的门户，DF/HCC成员可以通过该门户访问技术、试剂、 服务，科学咨询。核心任务是支持尽可能广泛的功能 基因组学筛选项目；迅速传播有关新的功能基因组学方法的信息；以及 帮助研究人员从概念和分析开发到高通量筛选、分析和 验证。

Collaborative Functional Genomics Shared Resource Project Summary / Abstract Understanding the basic biological mechanisms that underlie the development and progression of cancer is fundamental to finding better approaches to cancer prevention and treatment. Loss-of-function genetic approaches, such as high-throughput RNA interference (RNAi) and CRISPR/Cas9, as well as gain-of-function approaches such as open reading frame (ORF) over-expression and CRISPR-activation (CRISPR-a) provide cutting-edge strategies to identify new cancer-related genes and pathways. The Collaborative Functional Genomics Core offers a single portal through which DF/HCC members gain access to technologies, reagents, services, and scientific consultation. The Core mission is to support the widest possible range of functional genomics screening projects; rapidly disseminate information about new functional genomics approaches; and help researchers navigate from concept and assay development to high-throughput screening, analysis, and validation.