Core 2: Biospecimen/Pathology Core

项目总结 这一共享资源的目的是提供人体组织、生物液和专家 在专家的指导下对所有项目的调查人员进行病理解释 病理学家。组织病理学和组织/生物体液资源已经存在 自1986年以来，在GI孢子的支持下扩大。截至今年，资源 包括3156例结直肠癌切除标本，363例结直肠腺瘤 结直肠癌肝转移430例，胰腺癌1204例 标本，结直肠癌移植瘤843例，胰腺癌移植瘤101例，6085例 来自结直肠癌高危人群的血液样本，以及2122个血液样本 来自胰腺癌患者。冰冻福尔马林固定石蜡包埋组织 绝大多数切除的材料、临床和结果数据都是可用的 标本。这个资源包括高质量的冰冻和福尔马林固定的收藏 来自125名快速尸检参与者的终末期胰腺癌或结直肠癌样本。 目前有911个组织芯片，其中133个是结直肠和胰腺的组织微阵列 肿瘤和疾病。此外，超过16个完全特征化的胰腺癌细胞 从人类肿瘤中制备了包括8个家族性胰腺癌细胞在内的细胞系 并与研究界广泛分享。这些生物样本被收获并储存起来 根据国家癌症研究所的生物信息库最佳实践指南。 将这些标本分发给孢子研究人员已在#年出版了120多篇文章。 在过去的5年里。这一核心获得了更多的异种移植，新鲜冷冻的结直肠和 胰腺肿瘤以及血液和胰液样本，并提供专家病理学 咨询调查人员。标本是在病理学家的监督下收集的。 与临床密切合作，在结直肠和胰腺肿瘤方面具有专业知识 这些领域的专家以及与基础研究调查人员同样密切的合作，以 最大限度地提高项目的翻译效果。我们维护基于Web的密码保护 我们在这个核心的组织追踪系统。这个基于Web的界面遵循 国家研究委员会的建议，并包括用户身份验证， 加密、审核跟踪和灾难恢复。已经建立了确定优先顺序的机制 向约翰·霍普金斯大兵内外的调查人员分配所需资源 癌症孢子。生物样本已经与其他50多个机构的调查人员共享。

PROJECT SUMMARY The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 3156 banked colorectal carcinoma resection specimens, 363 colorectal adenoma samples, 430 hepatic metastases of colorectal carcinoma, 1204 pancreatic carcinoma specimens, 843 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 6085 blood specimens from individuals at risk for colorectal carcinoma, and 2122 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. This resource has collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 125 rapid autopsy participants. Presently there are 911 tissue microarrays of which 133 are of colorectal and pancreatic neoplasms and diseases. In addition, over 16 fully characterized pancreatic carcinoma cell lines, including 8 familial pancreatic cancer cell lines have been prepared from human tumors and shared broadly with the research community. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 120 publications in the past 5 years. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. We maintain a password protected web-based tracking system for our tissues in this core. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions.