Flow Cytometry Shared Resource

流式细胞仪共享资源：项目总结 流式细胞仪共享资源(FCSR)提供了对流式细胞仪分析和细胞分类的访问 为赫伯特·欧文综合癌症中心(HICCC)成员提供仪器和服务。在.之下 作为Remi Creusot博士的领导，FCSR提供：(1)多色面板流动数据采集和分析 使用流式细胞仪分析仪，包括高通量筛选(HTS)、(2)流式细胞仪细胞分选(4- 路或六路或板)由FCSR的专用操作员使用高速细胞分选器，包括单细胞索引 分类，以及(3)全面咨询、小组设计、实践培训、故障排除和数据分析 由博士级别的FCSR科学家提供的服务。这一广泛和包容的一揽子服务使HICCC能够 研究人员分析肿瘤细胞种群及其微环境，包括肿瘤浸润性 单个细胞水平的淋巴样细胞和髓样细胞。在当前项目期间(2014-2019年)，FCSR有 已经与哥伦比亚翻译免疫学中心(CCTI)的流式细胞仪核心集成在一起。这个 这些设施的合并确保了对更广泛的最先进仪器的访问，并带来了 为HICCC流式细胞仪使用者的研究提供先进的免疫学专业知识。HICCC成员现在拥有 可使用9台仪器，包括5台分析仪和4台分选机。此外，现有的两个 仪器/分析器已升级为18-20个探测器。此外，还购置了一台新的流入分拣机 扩大分拣服务能力。咨询服务的升级进一步增强了 HICCC成员从面板设计、协议面对面咨询中受益的研究 数据采集、分析和试验性故障排除方面的优化和实践培训 最大限度地发挥新的先进仪器对高维多色面板的影响。基于ITS 先进的仪器设备、高素质的员工、广泛的专业服务组合、易于访问以及 作为高性价比的服务，FCSR是HICCC成员首选的流式细胞仪服务提供商。这个 FCSR将继续采用最新技术，提供高质量的细胞分选、分析和 为HICCC成员提供咨询服务，以加深他们对癌症生物学的了解，并使新的 癌症治疗方面的突破。在当前项目期内，FCSR的能力已得到利用 由81个HICCC成员提供的关键数据和见解，支持了46个以上HICCC成员的成功 同行评议的出版物，包括在影响因子为10的期刊上发表的23篇稿件，其中12篇是 具有影响因子的期刊&gt；20(自然、细胞干细胞、癌细胞、癌症发现)，目前支持 NIH资助的21项研究资助(11项来自NCI)。

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY The Flow Cytometry Shared Resource (FCSR) gives access to flow cytometry analysis and cell sorting instruments and services to Herbert Irving Comprehensive Cancer Center (HICCC) members. Under the leadership of Remi Creusot, PhD, the FCSR provides: (1) multi-color panel flow data acquisition and analysis using flow cytometry analyzers, including high throughput screening (HTS), (2) flow cytometry cell sorting (four- way or six-way or plate) using high speed cell sorters by dedicated operators of FCSR, including single cell index sorting, and (3) comprehensive consultation, panel design, hands-on training, troubleshooting, and data analysis services by PhD-level FCSR scientists. This broad and inclusive package of services enables HICCC investigators to analyze tumor cell populations and their microenvironment, including the tumor-infiltrating lymphoid and myeloid cells at the single cell level. During the current project period (2014-2019), the FCSR has been integrated with the flow cytometry core of the Columbia Center for Translational Immunology (CCTI). The merging of these facilities has secured access to a broader range of state-of-the-art instrumentation and brought advanced expertise in immunology to the research of HICCC flow cytometry users. HICCC members now have access to nine instruments, including five analyzers and four sorters. In addition, two of the existing instruments/analyzers have been upgraded to 18-20 detectors. Moreover, a new Influx sorter has been acquired to expand the capacity of sorting services. Upgrades in consultation services have further empowered the research of HICCC members who now benefit from face-to-face consultation on panel design, protocol optimization, and hands-on training on data acquisition, analysis, and experimental troubleshooting essential to maximize the impact of new advanced instruments for high dimensional multi-color panels. Based on its advanced instrumentation, highly qualified staff, broad portfolio of specialized services, easy accessibility, and cost-effective services, the FCSR is the flow cytometry service provider of choice for HICCC members. The FCSR will continue to incorporate the latest technologies and to provide high quality cell sorting, analytical and consultation services to HICCC members to empower their understanding of cancer biology and to enable new breakthroughs in cancer therapy. Over the current project period, the capabilities of the FCSR have been utilized by 81 HICCC members, provided key data and insights to support the success of more than 46 HICCC member peer-reviewed publications, including 23 contributions in journals with impact factor >10, of which 12 were in journals with an impact factor >20 (Nature, Cell Stem Cell, Cancer Cell, Cancer Discovery), and currently support research for 21 NIH-funded research grants (11 from NCI).