Digital Extra Parameter (DxP8)

描述（由申请人提供）：我们正在申请资金，用于对现有BD Biosciences FACSCalibur分析流式细胞仪进行Cytek Development DxP 8色升级。将升级的仪器是2005年微生物学和免疫学系购买的共用核心设备。它目前具有标准的2激光和4色配置，因此允许多达4种颜色的荧光分析，荧光染料和荧光蛋白的范围相对有限。该部许多实验室广泛使用这一仪器，仪器保养良好，工作状况良好。然而，由于该部许多调查人员进行的流式细胞仪分析越来越复杂，这种相对简单的流式细胞仪已不能满足目前的需要。解决此问题的一个经济有效的解决方案是购买和安装本应用程序要求的Cytek数字8色升级。Cytek Development是一家成熟的公司，专门从事流式细胞仪的维修，维护，翻新和升级。我们有丰富的经验，该公司可以追溯到5年多，我们的经验，他们的产品和服务，使我们相信，拟议的8色升级将是一个具有成本效益的解决方案，我们目前需要一个更先进的流式细胞仪。微生物学和免疫学系至少有13名由国家卫生研究院资助的教员已被确定为该设备的用户，而且很可能还会从该系16名小学教员和19名中学教员管理的实验室中确定更多的用户。该设备的使用和维护将由本申请的PI Steven A博士监督。Porcelli是微生物学和免疫学系教授，也是爱因斯坦校区所有共享流式细胞术资源的总体科学总监。一名具有维护和操作FACSCalibur流式细胞仪经验的技术人员和一些专家顾问可确保升级后的FACSCalibur的功能得到充分利用。DxP8 8颜色升级将为大量NIH资助的微生物学家和免疫学家提供先进的流式细胞术技术，这些技术对广泛的基础和转化研究项目至关重要。

DESCRIPTION (provided by applicant): We are requesting funds for a Cytek Development DxP 8-color upgrade for an existing BD Biosciences FACSCalibur analytical flow cytometer. The instrument to be upgraded is shared core equipment that was purchased by the Department of Microbiology and Immunology in 2005. It currently has the standard 2 laser and 4 color configurations, and thus allows up to 4 color fluorescence analyses with a relatively restricted range of fluorochromes and fluorescent proteins. This instrument is used extensively by many of the laboratories in the Department, and has been well maintained and is in excellent working order. However, because of the increasing complexity of FACS analyses done by many of the Department's investigators, this relatively simple flow cytometer is no longer able to meet current needs. A cost-effective solution to this problem is the purchase and installation of the Cytek digital 8 color upgrade requested by this application. Cytek Development is a well established company that specializes in the repair, maintenance, refurbishing and upgrading of flow cytometers. We have extensive experience with this company going back more than 5 years, and our experience with their products and services makes us confident that the proposed 8 color upgrade will be a cost- effective solution to our current need for a more advanced flow cytometer. At least 13 NIH funded faculty in the Department of Microbiology and Immunology have been identified as users for this equipment, and it is likely that additional users will be identified from laboratories run by the Department's 16 primary and 19 secondary faculty members. The use and maintenance of this equipment will be supervised by the PI of this application, Dr. Steven A. Porcelli, who is Professor in the Department of Microbiology and Immunology and overall Scientific Director of all of the shared flow cytometry resources on the Einstein campus. A technician with experience in maintaining and operating FACSCalibur flow cytometers and a number of expert consultants are available to ensure that the features of the upgraded FACSCalibur will be used to full advantage. The DxP8 8 color upgrade will provide a large group of NIH-funded microbiologists and immunologists with access to advanced flow cytometry techniques that are critically important to a wide range of basic and translational research projects.