Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

项目摘要/摘要 耶克斯国家灵长类动物研究中心(YNPRC)的艾滋病研究组合继续扩大 非人灵长类(NHP)的增长约为1200万美元(57%)就是明证。 过去五年的艾滋病研究拨款。赠款资金的增长伴随着高额的 本研究的动物模型为无特定病原体(SPF)的印度原产猕猴。 YNPRC维护了一群SPF恒河猴(以前有U24支持)，以提供 这些动物用于艾滋病毒/艾滋病研究。此应用程序请求继续支持(现在是U42机制)以 通过最大化生产来扩大SPF群体。应用程序包括概述、畜牧业和 管理核心；病毒检测核心；以及MHC基因分型核心。这笔赠款将支持一个子集 整个YNPRC SPF群体的收入，并将从动物分配费和按日收费中获得计划收入。 YNPRC将提供机构支持，以支付这个殖民地以及其余殖民地的剩余费用 SPF菌落的。 管理和畜牧业：利用U42提供的资源，最大限度地提高 并为NIH资助的艾滋病毒/艾滋病研究分配这些动物提供便利。 病毒检测：提供血清学和分子病毒诊断检测，以支持SPF菌落，并 在耶克斯NPRC保持一个没有影响艾滋病相关研究的疾病的群体。 MHC基因分型：提供亲子鉴定，MHC I类和II类的综合分析 等位基因，并开发详细的基因特征的新技术，以支持基因健康的 繁育群体，并提供动物遗传信息，这些信息对动物分配过程至关重要 支持艾滋病研究。 实现这些目标将确保Yerkes NPRC SPF育种计划是可持续的，提供 使用印度血统的恒河猴来支持我们的艾滋病毒/艾滋病研究计划所需的动物。这个 使用恒河猴模型进行临床前研究的科学家可获得这一关键资源 将对预防或治愈艾滋病毒感染的治疗方法的发展产生积极而重大的影响 在人们身上。

Project Summary/Abstract The AIDS research portfolio at Yerkes National Primate Research Center (YNPRC) has continued to expand as demonstrated by a growth of approximately $12 million dollars (57% increase) in nonhuman primate (NHP) AIDS research grant funding over the past five years. This growth in grant funding is accompanied by a high demand for Indian-origin specific pathogen free (SPF) rhesus macaques as the animal model for this research. YNPRC has maintained a colony of SPF rhesus macaques (previously with U24 support) in order to provide these animals for HIV/AIDS research. This application requests continued support (now a U42 mechanism) to expand the SPF colony by maximizing production. The application includes the Overview; Husbandry and Management Core; the Viral Testing Core; and the MHC Genetic Typing Core. This grant will support a subset of the overall YNPRC SPF colony and will derive program income from animal assignment fees and per diems. YNPRC will provide institutional support to cover remaining expenses for this colony as well as the remainder of the SPF colony. Management and Husbandry: To leverage the resources provided by the U42 to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. Viral Testing: To provide serological and molecular viral diagnostic testing in support of the SPF colony and to maintain a colony free of diseases that impact AIDS-related research at the Yerkes NPRC. MHC Genetic Typing: To provide parentage testing, comprehensive analysis of MHC class I and class II alleles, and to develop new techniques for detailed genetic characterization to support a genetically healthy breeding colony and provide genetic information on animals essential to the animal assignment process in support of AIDS research. Achieving these aims will ensure the Yerkes NPRC SPF breeding program is sustainable, providing the necessary animals to support our HIV/AIDS research program using Indian-origin rhesus monkeys. The availability of this critical resource to scientists performing preclinical studies using the rhesus monkey model will have a positive, significant impact on the development of treatments to prevent or cure infection from HIV in people.