IMAGING CORE

C.成像核心计划 数字图像采集和分析是该中心研究目标的关键。目前有 15名中心研究者及其研究团队积极使用许多共享硬件/软件 图像采集和分析系统。计算机辅助图像处理的应用现状 包括1）使用交钥匙系统，如共聚焦显微镜，或 通过光学和电子显微镜进行图像采集的数字显微镜; 2）使用交互式 自动提取二维和三维定量信息的“计算机辅助解剖学”系统 3）定制软件包，用于从组织学切片进行三维重建和渲染， 串行图像堆栈有助于可视化和理解复杂的形态学关系， 和4）软件开发，用于在新兴领域中创建图像分析范例， 脑功能成像 成像核心的目标是为中心提供所需的专业知识和技术支持 研究者将充分受益于这一快速变化的硬件/软件中提供的研究工具 环境，并在可行的情况下，向更广泛的科学界传播在此开发的成像工具。 为了实现这一目标，Imaging Core将在多个层面提供支持。 目标1（图像采集）提供设施维护、用户培训和/或图像采集 新的共享共焦显微镜和共享数字图像采集系统的服务， 电镜 目标2（图像分析和处理）解决了相关的集中基础的需求 图像分析和软件开发方面的知识和专业知识，以帮助1）软件匹配 应用程序与研究目标时，一个很好的匹配存在，2）定制的软件需要时， 现有的应用程序或应用程序组可以修改以适应特定的需求，以及3）重新启动 当没有其他好的解决方案时，开发图像处理软件工具。 目标3（传播）建议进一步开发虚拟教学和研究工具， 继续赞助建立一个网站，使广大科学界能够查阅这些资料。

C. Imaging Core Plan Digital image acquisition and analysis are key to the research goals of the Center. There are currently 15 Center Investigators and their research teams actively using a number of shared hardware/software systems for image acquisition and analysis. Current applications of computer-assisted image processing span a wide range of complexity including 1) use of turn-key systems such as confocal microscopy or digital photomicroscopy for image acquisition through light and electron microscopes; 2) use of interactive "computer-aided anatomy" systems for automating the extraction of quantitative information in 2-D and 3-D from histological sections; 3) customization of software packages for 3-D reconstruction and rendering from serial image stacks to aid in the visualization and understanding of complex morphological relationships, and 4) software development for the creation of image-analysis paradigms in the emerging field of functional brain imaging. The goals of the Imaging Core are to provide the expertise and technical support required for Center Investigators to derive full benefit from the research tools available in this fast-changing hardware/software environment and to disseminate, where practicaj, the imaging tools developed here to the wider scientific and clinical community To achieve this, the Imaging Core will provide support at several levels. Aim 1 (image acquisition) provides facilities maintenance, user training and/or image-acquisition services on the new shared confocal microscope and shared digital-image acquisition systems for light and electron microscopy. Aim 2 (image analysis and processing) addresses needs for a centralized base of relevant knowledge and expertise in image analysis and software development to aid in 1) the matching of software application with research goals when a good match exists, 2) the customization of software required when existing applications, or groups of applications, can be modified to fit particular needs and 3) the de-novo development of image-processing software tools when no other good solution exists. Aim 3 (dissemination) proposes the further development of virtual teaching and research tools and the continued sponsorship of a website to make them available to the greater scientific community.