800 MHz NMR Spectrometer for the Central Alabama High-Field NMR Facility

描述（由申请人提供）：一个多机构用户组正在向NCRR-HEI项目申请800 MHz核磁共振光谱仪和CryoProbe的资金，以建立nih - ncrr支持的“阿拉巴马中部高场核磁共振设施”，作为进行生物分子研究的共享资源。用户组来自阿拉巴马州和密西西比州的六个机构，其中包括四个主要的博士学位授予机构（阿拉巴马大学伯明翰分校（UAB），奥本大学，阿拉巴马大学（塔斯卡卢萨）和密西西比大学）和两个主要的本科机构（塔斯基吉大学和密西西比学院）的教员。该设施将位于领导机构UAB的校园内，这对于其他机构的教职员工来说是方便的，并且为多机构用户群体的共享资源的运营和管理提供了基础设施。UAB管理局和几个部门和中心也在空间和初始运营成本方面对这个拟议的高场核磁共振设施做出了重大承诺。基于trosy的脉冲序列在800 MHz线窄化时效率的提高，将使某些机构无法在现有的600 MHz和500 MHz核磁共振技术下对较大的蛋白质和蛋白质复合物进行结构研究。更高的灵敏度（通过CryoProbe进一步显著增强）和800 MHz的化学位移色散将使微量化合物，少量可溶性蛋白质和复杂碳水化合物的研究成为可能，这些通常受到共振重叠的困扰。这个800兆赫的核磁共振系统将是拟议的阿拉巴马州中部高场核磁共振设施的中心部分，并将对美国国立卫生研究院支持的研究项目以及阿拉巴马州和密西西比州的其他项目产生重大影响。公共卫生相关性：拟议的800 MHz核磁共振仪器将提供尖端的研究能力，这对许多在疾病中起重要作用的生物分子的研究至关重要。它也将在低分子量天然产物的表征和具有治疗这些疾病的治疗潜力的新化合物的基于结构的设计方面具有重要意义。

DESCRIPTION (provided by applicant): A multi-institutional user group is requesting funding from the NCRR-HEI Program for an 800 MHz NMR spectrometer with a CryoProbe to establish an NIH-NCRR-supported "Central Alabama High-Field NMR Facility" as a Shared Resource to conduct biomolecular research. The user group is drawn from six institutions in Alabama and Mississippi, and includes faculty members from four major PhD granting institutions (the University of Alabama at Birmingham (UAB), Auburn University, the University of Alabama (Tuscaloosa), and the University of Mississippi) and two predominantly undegraduate institutions (Tuskegee University and Mississippi College). The facility will be physically located on the campus of the lead insitution, UAB, which is conveniently located to the faculty members from the remaining institutions, and has the infra-structure in place for the operation and management of this Shared Resource for the multi-institutional user group. The UAB Administration and several Departments and Centers have also made a significant commitment to this proposed High-Field NMR Facility in terms of space and the initial operating costs. The increased efficiency at 800 MHz in line narrowing for TROSY-based pulse sequences will enable structural investigation of larger proteins and protein complexes not amenable at existing 600 MHz and 500 MHz NMR technology at some of these institutions. The higher sensitivity (further significantly enhanced by the CryoProbe) and chemical shift dispersion at 800 MHz will enable investigations on trace quantities of compounds, sparingly soluble proteins, and complex carbohydrates which are often plagued by resonance overlap. This 800 MHz NMR system will be the center piece of the proposed Central Alabama High-Field NMR Facility and will have a significant impact on NIH- supported research projects as well as other projects in the States of Alabama and Mississippi. PUBLIC HEALTH RELEVANCE: The proposed 800 MHz NMR instrument will provide cutting-edge research capability that is critical for a number of investigations on biomolecules that play a significant role in diseases. It will also be significant in the characterization of low molecular weight natural products and the structure-based design of new compounds with therapeutic potential in treating these diseases.