HOPKINS DIGESTIVE DISEASE BASIC RESEARCH DEVELOPMENT CENTER

概述（由申请人提供）：霍普金斯消化疾病基础研究发展核心中心是NIDDK研究发展中心（DDBRDCC），旨在推进约翰霍普金斯大学医学院的基础科学和转化消化疾病研究，并通过向其成员和准成员提供核心，促进消化（胃肠道和肝脏）疾病研究的合作和新方向：A.上皮蛋白组学核心；B.成像芯；C.小鼠生理学核心。DDBRDCC是联合husom和该中心成员之间的伙伴关系，联合husom在人员和设备方面的投资补充了该中心的预算。核心A，上皮蛋白组学核心将帮助识别在Gl系/肝/肾正常或在这些器官的疾病中差异表达的蛋白质，跟踪蛋白质之间的相互作用，包括鉴定结合伙伴，检查蛋白质转换，绘制切割位点和确定翻译后修饰。该方法将使用二维和液相色谱分离和质谱。该核心将提供一名技术人员与核心调查人员进行规划、质量控制和生物信息学咨询。Core B， Imaging Core将为DDBRDCC研究人员提供多种类型的前沿上皮细胞成像和EM，并提供Core Manager来帮助进行研究。这些包括共聚焦和双光子显微镜；使用荧光计、数码相机；以及使用激光捕捉显微镜。Core B还将协助DDBRDCC研究人员为EM准备肠道、肝脏、胰腺和肾脏。Core C，小鼠生理学核心将在建立小鼠菌落、消化疾病小鼠基因型模型、提供代谢笼以及进行尿液、粪便、血液收集和分析方面提供建议。该核心还将进行小鼠的主动脉灌注，作为肠道、肝脏、胰腺、肾脏的一部分，为北方和西方的分析做准备。这种方法也将被Core C用于去除、定向、固定、处理、嵌入、切片和安装这些组织，以用于光学显微镜。Core C将为使用腔室/电压钳/活性肠道Na+和Cl-转运研究提供设备和使用说明。行政业务部将提供DDBRDCC的沟通、财务管理和行政职能，并组织浓缩方案（由非核心来源提供资金）。

DESCRIPTION, OVERALL (provided by applicant): The Hopkins Digestive Diseases Basic Research Development Core Center is an NIDDK Research Development Center (DDBRDCC) which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine and to foster collaborations and new directions of digestive (gastrointestinal and liver) diseases research by providing access for its members and associate members to Cores: A. Epithelial Proteomics Core; B. Imaging Core; C. Mouse Physiology Core. The DDBRDCC is a partnership between the JHUSOM and the members of this Center, with investment in personnel and equipment by the JHUSOM supplementing the Center budget. Core A, Epithelial Proteomics Core will help identify proteins that are differentially expressed in Gl tract/liver/kidney normally or in diseases of these organs, track interactions among proteins including identification of binding partners, examine protein turnover, map cleavage sites and determine post-translational modifications. The approaches will use 2D and liquid chromatography separations and mass spectrometry. This Core will provide a technician to interface with Core investigators for planning, quality control, and bioinformatics consultation. Core B, Imaging Core will make multiple types of cutting edge imaging and EM of epithelial cells available to DDBRDCC investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy; use of a fluorometer, digital camera; and access to laser capture microscopy. Core B will also assist DDBRDCC investigators prepare intestine, liver, pancreas and kidney for EM. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies, genotype mouse models of digestive diseases, make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver, pancreas, kidney procurement and preparation for Northern and Western analysis. This approach will also be used by Core C to remove, orient, fix, process, embed, section and mount these tissues to be used for light microscopy. Core C will make equipment available and instruction in its use for Ussing chamber/voltage clamp/active intestinal Na+ and Cl- transport studies. Administrative Operations will provide the communication, financial management and administrative functions of the DDBRDCC and organize the enrichment program (financed by non-Core sources).