Cells and Tissue Core

细胞和组织芯 建立细胞和组织核心以提供用于分化气道模型的气道细胞 和体外基因转移实验。使用这些模型是理解 CF的病理生理学和CFTR基因转移到气道上皮的研究。 总体目标： 该核心的目的是提供对非CF和CF组织和气道细胞的集中访问，用于 模型系统，以评估基因转移到气道和病理生理学在CF。 核心小组的主要职责是： 1.采集正常和CF组织; 2.人气道上皮细胞体外模型的建立 3.该中心成员通过基因转移对CF气道上皮进行电生理分析; 4.小鼠、雪貂和猪气道上皮细胞体外模型的建立; 5.气道上皮细胞培养与研究新方法的研究与开发 3.开发作为上皮生长的气道上皮细胞系（人、猪和雪貂）; 7.创建CF肺组织采集的气道组织和原代细胞培养物库 方案; 8. CF和非CF肺组织和RNA的低温库的开发; 9.向研究者讲授体外模型系统的开发方法; 10.获得IRB监管委员会的批准并保存记录。 一个成功的核心将提供模型和专业知识，以不断增长的社区调查，从 他们在不同的领域，并允许他们专注于CF基因转移的发展独特的专业知识。

Cells and Tissue Core The Cells and Tissue Core was established to provide airway cells for use in differentiated airway models and in vitro gene transfer experiments. The use of these models is required for understanding the pathophysiology of CF and for studies of CFTR gene transfer to airway epithelia. Overall Objective: The purpose of this core is to provide centralized access to non-CF and CF tissue and airway cells used for model systems to assess gene transfer to the airway and pathophysiology in CF. The main responsibilities of the Core will be: 1. Acquisition of normal and CF tissue; 2. Generation of in vitro models of human airway epithelia; 3. Electrophysiologic analysis of CF airway epithelia transduced by gene transfer by members of the Center; 4. Generation of in vitro models of mice, ferrets and pig airway epithelia; 5. Research and development on new methods for the culture and study of airway epithelia; 3. Development of airway epithelia cell lines (human, pig and ferret) that grow as epithelia; 7. Creation a repository of airway tissue and primary cell cultures form the CF Lung Tissue Acquisition Program; 8. Development of a cryogenic bank of CF and non-CF lung tissue and RNA; 9. Teaching investigators the methods for developing in vitro model systems; 10. Obtaining approval and record keeping for IRB regulatory committees. A successful Core will provide the models and expertise to the growing community of investigators from different areas, and allow them to focus their unique expertise on the development of CF gene transfer.