10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology

描述(申请人提供)：本申请用于资助研究生和博士后参加第十届细胞色素P450生物多样性和生物技术国际研讨会。本次会议涉及细胞色素P450基因和酶的进化、结构、功能和应用，重点介绍了细胞色素P450在细菌、真菌、植物和非哺乳动物动物模型中的细胞色素P450。晶体结构的数量迅速增加，再加上大量的CYP序列(现在超过12,000个)，使得从序列和三级结构的角度来看待结构演变成为可能，比以前更有可能理解。蛋白质结构研究考虑了CYP酶在生物活性化合物合成、外源化学物质和内源化合物代谢中的作用。这次会议还包括CYP基因的工程，以产生具有新功能的酶，以及深入了解外源和内源底物的催化机制。还讨论了信息和工程系统在生物修复、风险评估和生态过程中的应用。会议包括每个分类组对外来生物暴露的反应，以及CYP基因和蛋白质在适应和响应该暴露中的作用。这将包括毒理学中使用的重要非哺乳动物动物模型，如斑马鱼和其他动物。 公共卫生相关性：本申请用于资助研究生和博士后参加第十届细胞色素P450生物多样性和生物技术国际研讨会。这次会议将为广泛的微生物、真菌、昆虫、植物和一些脊椎动物物种的细胞色素P450(CYP)基因和蛋白质的讨论提供一个重要的论坛。第十届研讨会的总体目标是促进对细胞色素P450(CYP)结构和功能演变的理解的综合，以及在环境评估、修复和新潜在药物合成中应用这些知识的新方向。这些目标将通过提供一种机制来获得来自杰出科学家的新想法和投入，这些科学家代表了非常不同的专业知识和培训背景，处于细胞色素P基因研究的前沿。本次会议包括现有P450结构之间的进化和机制比较，微生物、昆虫和植物P450在合成和解毒途径中的功能比较，以及用于技术和环境应用的P450催化部位的生物工程。研究生和博士后的参与对于这一重要研究领域的持续活力非常重要。

DESCRIPTION (provided by applicant): This application is for funding for graduate students and post-docs to attend the 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology. This meeting deals with aspects of evolution, structure, function, and application of cytochrome P450 genes and enzymes, emphasizing CYPs in bacteria, fungi, plants, and non-mammalian animal models. The rapidly increasing number of crystal structures coupled with the huge number of CYP sequences (now in excess of 12,000), makes it possible to look at structural evolution from the sequence as well as tertiary structural perspective, with a greater understanding than possible before. Protein structural studies consider function of CYP enzymes in synthesis of bioactive compounds, and in the metabolism of xenobiotic chemicals and endogenous compounds. This meeting also includes engineering of CYP genes, to generate enzymes with novel functions as well as to gain insights into catalytic mechanisms with xenobiotic and endogenous substrates. The application of information and engineered systems in bioremediation, risk assessment and ecological processes is also addressed. The meeting includes responses of each taxonomic group to xenobiotic exposure, and the role of CYP genes and proteins in adaptation and response to that exposure. This will include important non-mammalian animal models used in toxicology, such as zebrafish and others. PUBLIC HEALTH RELEVANCE: This application is for funds to provide support for graduate students and post-doctoral students to participate in the 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology. This meeting will provide a critical forum for discussions on cytochrome P450 (CYP) genes and proteins a wide range of microbial, fungal, insect, plant and some vertebrate species. The overall goal of this 10th symposium is to foster a synthesis of understanding of cytochrome P450 (CYP) structural and functional evolution, and new directions in application this knowledge in environmental assessment, remediation, and synthesis of new potential drugs. The goals will be accomplished by providing a mechanism for garnering new ideas and input from outstanding scientists that represent a very diverse background of expertise and training, at the forefront of research on CYP genes. Sessions in this meeting include evolutionary and mechanistic comparisons among available P450 structures, functional comparisons among microbial, insect and plant P450s in synthetic and detoxification pathways, as well as bioengineering of P450 catalytic sites for technological and environmental applications. Participation of graduate students and post-doctoral students is important for a continued vitality in this important area of research.