AXEL BRUNGER PRT TIME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拟议的实验涉及对小蛋白质晶体的表征。所讨论的蛋白质是由两种不同蛋白质组成的相当大(~500 kDa)的复合体。在结晶紫染料的存在下，晶体呈蓝色，表明它们是由蛋白质组成的。然而，晶体只能以小平板的形式生长，平板表面的尺寸约为100-200微米，与平板垂直测量的尺寸为50微米。对晶体进行研究，以确定衍射度、孪生和马赛克。还将研究低温条件的潜在改善。这些晶体已经在各种不同的基质中生长。还将研究这些衬底对衍射性质的影响。确定空间群和收集这些晶体的原生集合将是最终目标。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed experiments involve the characterization of small protein crystals. The protein in question is a fairly large (~500 kDa) complex of two different proteins. The crystals stain blue in the presence of crystal violet dye, indicating that they are composed of protein. However, the crystals grow only as small plates, with dimensions of approximately 100-200 microns on the plate face and 50 microns measured orthogonal to the face. The crystals are to be studied to determine extent of diffraction, twinning and mosaicity. Potential improvements in cryo-conditions will also be examined. The crystals have been grown in a variety of different substrates. The effect of these substrates upon the diffraction properties will be studied as well. Determination of space group and the collection of a native set for these crystals would be the ultimate goal.