SYNCHROTRON X-RAY STUDIES OF BIOMOLECULAR MATERIALS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这些实验旨在阐明控制细胞骨架丝状蛋白(生物聚合物神经丝、微管和丝状肌动蛋白)之间相互作用的关键参数，并导致纳米到微米级的分级超分子结构。生物聚合物组件将分别在粉末和定向样品中使用X射线毛细管和微通道进行表征。微通道中定向组装的同步加速器结构研究应该揭示交联物的有序性，或者在神经细丝的情况下，突出侧臂蛋白质的性质，并为细丝间相互作用的起源提供重要的见解。有什么建议？S的独特方法是将同步加速器X射线散射与实空间成像与电子和光学显微镜相结合，探测和解决生物大分子组装的非晶体结构(蛋白质结晶学技术不适用于此)。同步X射线散射在倒易空间产生统计平均结构。学习到的概念应该会导致开发先进的纳米级材料的令人兴奋的可能性，用于电子、化学和制药行业的纳米科学应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The experiments aim to elucidate the key parameters that control the interactions between cytoskeletal filamentous proteins (the biopolymers neurofilaments, microtubules, and filamentous actin), and lead to hierarchical supramolecular structures on the nanometer to micron scale. The biopolymer assemblies will be characterized in powder and oriented samples using x-ray capillaries and microchannels, respectively. The synchrotron structure studies of oriented assemblies in microchannels should reveal the nature of the ordering of the cross-linking, or, in the case of neurofilaments, protruding sidearm proteins, and provide important insight into the origin of interfilament interactions. The proposal?s unique approach combines synchrotron x-ray scattering, which yields statistically averaged structures in reciprocal space, and real-space imaging with electron and optical microscopy, to probe and solve non-crystalline structures of biomacromolecular assemblies (where protein crystallography techniques do not apply). The learned concepts should lead to the exciting possibility of developing advanced nanometer scale materials for nanoscience applications in the electronic, chemical, and pharmaceutical industries.