Viral Sequencing Core

描述Core J与研究中心研究者和CHAVI内其他人之间的互动，以解释 目标和这些不同群体协同工作的方式。 在每周的CHAVI会议上，定期讨论Core J研究人员生成的结果 电话会议。因此，临床研究中心和其他CHAVI研究者敏锐地意识到相关数据， 问题和问题，并就向前迈进进行协商。这种相互作用的例子， 过去一年的工作包括：（i）临床研究人员从长期受感染的病人身上抽取样本， 用于急性感染分析的对照，（ii）选择第一组功能性env克隆用于进一步的 生物学表征，（iii）选择急性感染样本的子集，以进行更详细的纵向研究 准种分析，以及（iv）选择急性感染样品用于CTL的全长基因组分析 问题研究 不同的测序中心如何监测性能指标（UAB和南非）？是谁 在这两个网站之间做什么 在过去的预算期内，南非的研究中心重点关注急性 和慢性亚型C感染，而UAB，杜克和杜克大学的网站已经完成了第一个全面的 急性B亚型感染分析。在未来的一年里，南非和坦桑尼亚将负责 为完成南非和马拉维急性C亚型感染的遗传特征鉴定， 而UAB和杜克将专注于急性感染样本的纵向分析， 序列分析和功能克隆的产生。优先事项和工作量是在一个 季度基础上。各个地点的进展情况由儿童和青少年健康问题委员会SLG进行审查，预算调整由 在必要时作出。

Describe the interactions between Core J and site investigators and others within CHAVI to explain the goals and the ways in which these various groups might work synergistically. Results generated by Core J investigators are discussed on a regular basis at the weekly CHAVI conference calls. Thus, clinical site and other CHAVI investigators are acutely aware of pertinent data, questions and issues, and are consulted with respect to moving forward. Examples of such interactions in the past year included: (i) selection of samples from chronically infected patients by clinical investigators as controls for the acute infection analysis, (ii) selection of a first set of functional env clones for further biological characterization, (iii) selection of a subset of acute infection samples for more detailed longitudinal quasispecies analyses, and (iv) selection of acute infection samples for full-length genome analysis for CTL studies. How are the different sequencing sites monitoring performance metrics (UAB and South Africa)? Who is doing what between these two sites? In the past budget period, the South African site has focused on the genetic characterization of acute and chronic subtype C infections, while UAB, Duke and UNC sites have completed the first comprehensive analysis of acute subtype B infections. In the coming year, South Africa and UNC sites will be responsible for the completion of the genetic characterization of acute subtype C infections in South Africa and Malawi, while UAB and Duke will focus on longitudinal analyses of acute infection samples, full length genome sequence analysis, and generation of functional clones. Priorities and workload are discussed on a quarterly basis. Progress at individual sites is reviewed by the CHAVI SLG and budgetary adjustments are made when necessary.