Analytical & Phenotyping Core

分析和表型分析核心的目的是提供交感压力反射功能的最先进的心血管和生化分析，包括交感神经流量、儿茶酚和去甲肾上腺素清除和溢出的测量。这一资源对于我们小组识别和描述涉及自主神经系统的新疾病至关重要，例如多巴胺-β-羟基酶缺乏症。在这个核心中建立了各种技术来支持单个项目，包括测定血浆容量(项目4)、心率和血压变异性(项目1、2、3和4)以及压力反射功能(项目1和4)。 尤其是项目2，在所有其他项目中，将利用交感神经活动的生化评估，这是对临床核心中详细说明的其他自主评估方法的补充。项目1、3和4将利用用于表型分析的心血管参数的超传统节律分析方面的专业知识。 项目1和4将使用我们针对神经信号的新型信号处理工具。此外，我们还实施了一种动物表型设备，将开发的用于评估人类自主神经功能的方法应用于将在项目1中主要使用的小鼠。这包括监测清醒的无限制小鼠的动脉内血压，监测肾交感神经活动，压力感受器反射测试，渗透压测试，以及高保真心电图，以及正常和去窦主动脉神经的小鼠。转基因小鼠在翻译自主神经方面越来越重要 知识，而这个表型小鼠实验室在这方面将是必不可少的。在本供资期间，核心将提供类似的生化分析和表型鉴定服务，但重点是人类数据，包括增强的信号处理、通过自动数据库馈送进行数据缩减、现有数据库之间的链接、为所有项目开发数据探索工具。

The purpose of the Analytical and Phenotyping Core is to provide state-of-the-art cardiovascular and biochemical analysis of sympathetic baroreflex function, including measurements of sympathetic nerve traffic, catechols, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such as dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume (Project land 4), heart rate and blood pressure variability (Project 1,2,3, and 4), and baroreflex function (Project 1 and 4). Especially Project 2 among all the others will utilize biochemical evaluation of sympathetic activity which complements other methods of autonomic evaluation detailed in the Clinical Core. Expertise in analysis of ultradian rhythms of cardiovascular parameters for phenotyping will be utilized in Project 1, 3, and 4. Project 1 and 4 will use our novel signal processing tools for neurological signals. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice which will be used mainly in Project 1. This includes, among other things, monitoring intra-arterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity, baroreflex testing, osmopressor tests, and high fidelity ECG, in intact and sinoaortic- denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mouse laboratory will be essential in this regard. In this funding period, the Core will provide similar biochemical analytical and phenotyping services but with focus on human data including enhanced signal processing, data reduction with automatic database feeds, links between existing databases, development of data exploration tools for all projects.