High performance liquid chromatography system

目前的拨款提案是关于更换80年代初购买的高效液相色谱仪（HPLC）。系统4个月前就坏了，因为太旧了，无法修复。替换零件在市场上已经买不到了。HPLC技术被我们的研究中心（森林生物学研究中心（CRBF））的成员集中使用，目前的情况危及了几个研究项目和几个研究生的培养。魁北克地区似乎没有这种系统，因为该地区（魁北克城市地区的医疗和其他研究中心）的所有HPLC系统都被密集使用。高效液相色谱是CRBF分析基础设施的重要组成部分。我们的研究中心有固定成员共享设备的政策（教授19人，副研究员16人，博士后8人，研究生130人，研究人员31人）。高效液相色谱的维护和用户的监督（学生培训等）由森林昆虫学研究实验室的E. Bauce（教授）和M. Charest（生物化学家）负责。除了满足CRBF成员的需求（分析糖、氨基酸、多酚类物质等）外，HPLC还用于与康考迪亚大学、新不伦瑞克大学、加拿大林业局、维斯克萨大学（巴西）、威斯康星大学、蒙特利尔大学等同事在森林昆虫学方面的合作研究项目。这些项目在很大程度上是由NSERC (Bauce-discovery grant; Quiring et al (NSERC- crdg)；Bauce (NSERC-CRDG)。在这些研究项目中，高效液相色谱用于分析树叶中的多酚类物质、氨基酸和可溶性糖。采用分馏收集器制备植物多酚类昆虫生物测定样品。

The current grant proposal is about replacing a High performance liquid chromatograph (HPLC)bought in the early eighties. The system broke 4 months ago and cannot be repaired because it is too old. The replacement parts are no longer available on the market.   The HPLC technology is intensively used by the members of our research center (Center of research in forest biology (CRBF)) and the current situation jeopardizes severals research projects and the training of severals graduate students. It appears that there is no availabity of such system in the region of Quebec because all HPLC systems in the region (Medical and other research centers of the Quebec city region) are intensively used. High performance liquid chromatography is an important element of the CRBF analytical infrastructure. Our research center has a politic of equipment sharing among all regular members (19 professors, 16 associated researchers, 8 post-doc, 130 graduate students, and 31 research professionals). The maintenance of the HPLC and the supervision (student training etc)of the users are under the responsabilty of E. Bauce (professor) and M. Charest (biochemist) of the forest entomology research laboratory. In addition to covering the needs of the CRBF members (analysis of sugars, amino acids, polyphenolics etc.), the HPLC is used in collaborative research projects in forest entomology with colleagues from Concordia University, University of New-Brunswick, Canadian Forestry Service, University of Viscosa (Brazil), University of Wisconsin, University of Montreal etc.  These projects are in a large part supported by NSERC (Bauce-discovery grant; Quiring et al (NSERC-CRDG); Bauce (NSERC-CRDG). In these research projects, HPLC is used to analyse tree foliage polyphenolics, amino acids and soluble sugars. Fraction collector is used to prepare samples for insect bioassay with plant polyphenolics.