An ultricentrifuge : a basic instrument for the isolation of clathrin-coated vesicles by subcellular fractionation in order to do functional proteomic analysis

魁北克大学奇库蒂米分校（UQAC）正在开展细胞生物学研究。细胞生物学研究细胞的结构和功能。研究细胞的功能是很重要的，因为细胞是我们身体的主要组成部分。它们的定位和功能受到高度调控。组织的变化可能导致系统失调，进而导致疾病的发展。在细胞内部，有不同的区室，其中含有参与细胞功能的蛋白质复合物。为了更好地了解蛋白质和细胞的功能，已经开发了诸如隔室分离之类的技术来研究这些隔室和蛋白质复合物。其中一项技术是超离心，它可以分离特定的隔室并研究它们的蛋白质含量。为了在UQAC建立我的研究计划，我需要购置一台超离心机，因为这是UQAC的一个新的研究领域，并且没有这样的设备。

The University of Quebec at Chicoutimi (UQAC) is developping cell biology research. Cell biology studies the structure and the function of cells. It is important to study the function of cells since there are the major part of our body. Their localization and their function are highly regulated. Change in their organization could lead to a dysregulation of the system and then to the development of diseases. Inside the cells, there are different compartments containing protein complexes involved in cell function. Techniques have been developped such as compartment isolation to study these compartments and protein complexes in order to better understand protein and cell functions. One of the technique is the ultracentrifugation that allow to isolate specific compartments and to study their protein contents. In order to establish my research program at UQAC, I need to acquire an ultracentrifuge since this is a new field of investigation at UQAC and there is no such equipment available.