Thin-layer chromatography - flame ionization / flame photometric dual detection system

我们正在申请一台薄层色谱仪，这是一种用于测量样品提取物中有机化合物的先进分析工具。我们要求的TLC仪器是独一无二的，因为它具有双重检测系统，包括用于碳化合物的火焰离子化检测器（FID）和用于含磷和/或硫化合物的火焰光度检测器（FPD）。这种TLC-FID/FPD仪器在世界上只有一家制造商，在北美只有一家供应商。我们建议使用这种仪器来测量在海洋食物网和水产养殖中至关重要的化合物。我们将确定中性脂质类，包括碳氢化合物，三酰甘油和甾醇，和极性脂质类。这台仪器将取代我们13年的旧TLC-FID，它一直在故障。新模型及其配置将使我们能够扩展到两个新的重要研究领域：磷脂类和硫脂。磷脂类是迄今为止大多数生物膜的主要脂质组分，并且硫脂是藻类的主要脂质组分。人们对膳食脂质在海洋生态系统和水产养殖中的重要性以及将海洋脂质用作生物标志物的兴趣越来越大。海洋脂质包括几种在结构和功能上有相当大差异的脂质类别。结构上的差异允许它们用作生物标志物。在生物标志物方法中，某些特征化合物被用于整合生物、物理和化学参数，这些参数定义了生态系统的状态。使用这些化合物，我们可以量化必需营养素和有害化合物的输入，我们可以追踪它们的途径。TLC-FID/FPD将支持几个项目，其中我们的任务是确定对动物生长至关重要或可用作海洋环境中发生过程的标志物的脂质化合物。纽芬兰食物网中的脂质测量是及时的，因为地面渔业的持续问题，对新渔业物种的兴趣，以及该地区水产养殖和油田开发水平的提高。

We are requesting a thin-layer chromatography (TLC) instrument, which is a sophisticated analytical tool used to measure organic compounds in sample extracts. The particular TLC instrument we are requesting is unique as it has a dual detection system consisting of a flame ionization detector (FID) for carbon compounds and a flame photometric detector (FPD) for compounds containing phosphorus and/or sulphur. There is only one manufacturer of this TLC-FID/FPD instrument in the world and only one supplier in North America. We propose to use this instrument to measure compounds that are critical in marine food webs and aquaculture. We will determine neutral lipid classes including hydrocarbons, triacylglycerols and sterols, and polar lipid classes. This instrument will replace our 13 year old TLC-FID which keeps breaking down. The new model and its configuration will be such as to allow us to also expand into two new, important areas of research: phospholipid classes and sulpholipids. Phospholipid classes are by far the major lipid components of most biological membranes and sulpholipids are major lipid components of algae. There is considerable and growing interest in the importance of dietary lipids in marine ecosystems and aquaculture, as well as in the use of marine lipids as biomarkers. Marine lipids comprise several lipid classes with considerable differences both in structure and function. The differences in structure permit their use as biomarkers. In the biomarker approach, certain signature compounds are used to integrate biological, physical and chemical parameters that define the status of an ecosystem. Using these compounds we can quantify inputs of essential nutrients and of deleterious compounds and we can trace their pathways. The TLC-FID/FPD will support several projects wherein our task is to determine lipid compounds that are either critical to animal growth or that can be used as markers of processes occurring in the marine environment. Measurement of lipids in Newfoundland food webs is timely given the continuing problems with the ground fishery, interest in new fishery species, and the increasing level of aquaculture and oil field development in this area.