Voltage clamp elecrophysiological set-up for cultured neurons

我的研究项目涉及中枢神经系统细胞再生的研究，以及学习和记忆的细胞机制。我在这些研究中使用的动物模型是软体动物，因为它的脑细胞很大，很容易接近，而且许多都被单独识别为已知的递质（例如多巴胺）。许多信号通路和神经元机制在脊椎动物和无脊椎动物之间是保守的，因此该模型的研究结果可能直接影响我们对高等神经系统的认识。我正在进行的研究包括在培养环境中记录脑细胞，以及完整动物和分离神经系统的记录。我的实验室目前有三个记录装置，但其中只有一个是用于记录培养细胞的。我的研究项目在培养神经元方面一直在稳步发展，现在我需要将我的一个记录设备转换为培养细胞。

My research program involves the study of regeneration of cells of the central nervous system, as well as cellular mechanisms underlying learning and memory. The animal model I use in these studies is the mollusc, Lymnaea stagnalis, as its brain cells are large, easily accessible and many are individually identified with known transmitters (e.g. dopamine). Many signaling pathways and neuronal mechanisms are conserved between vertebrates and invertebrates, so research findings from this model may directly influence our knowledge of higher nervous systems. The research I am conducting includes recordings from brain cells in a cultured environment, as well as from intact animals and isolated nervous systems. I currently have three recording set-ups in my laboratory, but only one of these is configured for recordings from cultured cells. My research program has been steadily progressing in the area of cultured neurons, and I now need to convert one of my recording set-ups for use with cultured cells.