Effect of environmental stress conditions on functional and structural properties of photosystem II

光系统II的结构和电子传递活性的调节受到环境因素的强烈影响，如光和不同污染物的存在，这些因素会产生抑制作用。在这个研究项目中，我们试图了解结构和功能是如何适应这些环境条件的。当光合装置暴露于重金属和除草剂引起的毒性作用时，未用于光化学的多余光能将通过光收集复合物的色素通过热耗散或形成活性氧而耗散。我们的假设是，在这些条件下，能量耗散机制将反映重金属和除草剂诱导的抑制机制的特异性。在我们的项目中，我们将利用不同种类的高等植物和藻类及其特定的突变体，研究光系统II相关的能量耗散过程的机制。我们的研究目的是研究强光下抑制剂、重金属和除草剂的作用关系和能量耗散机制。通过使用高度先进的光谱研究和生化方法，实验将在体外和体内条件下进行。所获得的结果将为解释植物在不同光照条件下如何生存和耐受污染物的毒性效应提供信息。这些结果将有助于了解一些污染物的抑制作用与光能耗散机制之间的相互作用。

The regulation of Photosystem II structure and electron transport activity is strongly affected by environmental factors such as light and the presence of different pollutants which cause inhibitory effects. In this research project, we attempt to understand in which way structure and functions are accommodated to cope with those environmental conditions. When the photosynthetic apparatus is exposed to toxic effects induced by heavy metals and herbicides the excess light energy not used in photochemistry will be dissipated via pigments of the light harvesting complex through thermal dissipation or formation of reactive oxygen species. Our hypothesis is that, under those conditions, the mechanism of energy dissipation will reflect the specificity of the inhibition mechanism induced by heavy metals and herbicides. In our project, by using different species of higher plant and alga and their specific mutants, we will investigate the mechanism of energy dissipation process associated with Photosystem II. Our research objective is to study the relationships between the effects of inhibitors, heavy metals and herbicides induced under strong light intensity and energy dissipation mechanisms. By using highly advanced spectroscopic studies and biochemical methods, experiments will be done in in vitro and in vivo conditions. Results obtained will provide information which can explain how plants can survive and tolerate pollutants toxicity effects under different light conditions. These results will contribute to fundamental knowledge concerning the interaction between inhibitory effects of some pollutants and light energy dissipation mechanism.