Incubator shaker and accessories

该仪器将主要用于细菌的受控培养，通常是大肠杆菌。对于提交申请的研究人员和在生物系工作的其他人来说，这是一个基本的程序，他们将可以使用孵化器摇床和配件。培养的细菌将被用于克隆编码滞育期间上调的蛋白质的cDNA，并用于表达野生型和突变的真核蛋白，然后进行纯化。这些蛋白质被用在几种体外分析中，以研究蛋白质的性质，如分子伴侣，并作为抗原产生抗体，用于蛋白质的定量和定位。培养箱摇床将用于在控制温度和曝气的情况下培养卤虫。将研究卤虫胚胎发育和滞育期间的基因表达和蛋白质合成，这是一种生理休眠和增强的应激耐力状态。蛋白质结构/功能关系和分子伴侣对滞育期间细胞存活的贡献是这项工作的重要组成部分。利用培养箱摇床的研究将对细胞、分子和发育生物学做出基础性贡献，这项工作有很多应用。对滞育的研究可能揭示昆虫如何抵抗压力，这在农业和林业中非常重要，这些生物作为害虫的影响因进入滞育并在恶劣环境条件下生存的能力而加剧。更好地理解滞育将促进卤虫在水产养殖中作为饲料的使用。麦克雷博士研究了小分子热休克蛋白HSP27在乳腺癌细胞转移中的作用。显然，通过使用这里寻求的孵化器摇床和配件，有可能做出对商业和健康有直接影响的发现。这些设备还将促进解决基本问题的体验，这些问题是NSERC支持的研究项目的基石。资助这项建议将使申请者在有效使用赠款资金和在几个生物学科进行现代实验方面处于一定的优势。

The instrumentation will be used primarily for the controlled culture of bacteria, generally Escherichia coli. This is an essential procedure for the researcher submitting the application and for others working in the Department of Biology who will have access to the incubator shaker and accessories. Cultured bacteria will be used in cloning cDNAs encoding proteins up-regulated during diapause, and for expression of wild type and mutated eukaryotic proteins which are then purified. These proteins are used in several in vitro assays to investigate the properties of proteins such as molecular chaperones and as antigens for production of antibodies used in protein quantification and localization. The incubator shaker will be used to grow Artemia under controlled temperature and aeration. Gene expression and protein synthesis during Artemia embryo development and diapause, a state of physiological dormancy and enhanced stress tolerance, will be studied. Protein structure/function relationships and the contribution of molecular chaperones to cell survival during diapause are important components of this work. The research employing the incubator shaker will make fundamental contributions to cell, molecular and developmental biology, and there are many applications of the work. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where the impact of these organisms as pests is exacerbated by the ability to enter diapause and survive harsh environmental conditions. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Dr. MacRae studies the role of HSP27, a small heat shock protein, in breast cancer cell metastasis. Clearly there is potential to make discoveries which have direct consequences to commerce and health through use of the incubator shaker and accessories sought herein. The equipment will also facilitate experiemnts addressing basic questions which are the cornerstone of research programs supported by NSERC. Funding this proposal will put the applicant at a definite advantage in the effective use of grant funds and the ability to conduct modern experimentation in several biological disciplines.