Effect of environmental stress conditions on functional and structural properties of photosystem II

光系统II结构和电子传递活性的调节受到环境因素的强烈影响，例如光和引起抑制效应的不同污染物的存在。在这个研究项目中，我们试图了解结构和功能是如何科普这些环境条件的。当光合器官暴露于重金属和除草剂诱导的毒性效应时，光化学中未使用的过量光能将通过热耗散或活性氧物质的形成经由捕光复合物的色素耗散。我们的假设是，在这些条件下，能量耗散机制将反映重金属和除草剂诱导的抑制机制的特异性。本课题以不同种类的高等植物和拟南芥及其突变体为材料，研究光系统II的能量耗散机制。本研究的目的是研究强光下抑制剂、重金属和除草剂的诱导效应与能量耗散机制之间的关系。通过使用高度先进的光谱研究和生物化学方法，将在体外和体内条件下进行实验。研究结果将为解释植物在不同光照条件下如何生存和耐受污染物毒性效应提供信息。这些结果将有助于了解一些污染物的抑制效应与光能耗散机制之间的相互作用。

The regulation of Photosystem II structure and electron transport activity is strongly affected by environmental factors such as light and the presence of different pollutants which cause inhibitory effects. In this research project, we attempt to understand in which way structure and functions are accommodated to cope with those environmental conditions. When the photosynthetic apparatus is exposed to toxic effects induced by heavy metals and herbicides the excess light energy not used in photochemistry will be dissipated via pigments of the light harvesting complex through thermal dissipation or formation of reactive oxygen species. Our hypothesis is that, under those conditions, the mechanism of energy dissipation will reflect the specificity of the inhibition mechanism induced by heavy metals and herbicides. In our project, by using different species of higher plant and alga and their specific mutants, we will investigate the mechanism of energy dissipation process associated with Photosystem II. Our research objective is to study the relationships between the effects of inhibitors, heavy metals and herbicides induced under strong light intensity and energy dissipation mechanisms. By using highly advanced spectroscopic studies and biochemical methods, experiments will be done in in vitro and in vivo conditions. Results obtained will provide information which can explain how plants can survive and tolerate pollutants toxicity effects under different light conditions. These results will contribute to fundamental knowledge concerning the interaction between inhibitory effects of some pollutants and light energy dissipation mechanism.