Development of endolysin-based biosensor for the detection of Lasteria bacteria contamination

开发一种灵敏、廉价的生物传感器来现场检测和定量食品和饮用水样品中的细菌是一个重要的科学挑战。目前的细菌检测方法速度慢、效率低，不能在疫情发生时及时采取行动，这会造成巨大的经济负担，并对人类健康构成严重威胁。食品工业对细菌的快速准确检测/监测有很大的需求。我们正专注于开发一种针对李斯特菌的便携式、实时和低成本的检测系统。这一新的系统利用基于噬菌体的产品来完成对李斯特菌的灵敏检测/捕获。在我们的检测系统中，我们将使用来自李斯特菌特异性噬菌体的细菌细胞壁结合蛋白(CBD)作为识别受体。李斯特菌与CBD的高亲和力/特异性结合将通过基于阻抗的传感设备进行检测。这一独特的组合将产生一种快速检测食品样本中李斯特菌的新工具。这项技术不仅限于食品工业中的细菌检测，而且在水资源、国土安全、环境污染和护理点监测等方面也有广泛的应用。

The development of a sensitive, inexpensive biosensor to detect and quantify bacteria on-site in food and drinking water samples is an important scientific challenge. Current methods for bacteria detection are slow, inefficient and inadequate for taking timely action in case of outbreaks, which can result in tremendous economic burden and pose a serious risk to human health. There is a great demand for rapid and accurate detection/monitoring of bacteria in the food industry. We are focusing on the development of a portable, real-time and low-cost detection system targeting Listeria. This novel system utilizes a bacteriophage-based product to accomplish sensitive detection/capturing of Listeria. We will employ bacterial cell wall-binding proteins (CBD) derived from Listeria-specific phages as the recognition receptor in our detection system. The high-affinity/specificity binding of Listeria to the CBD will be detected by an impedance-based sensing device. This unique combination will result in a novel tool for the rapid detection of Listeria in food samples. This technology is not limited to bacteria detection in food industry, but also has wide applications in water resources, homeland security, environment pollution and point-of-care monitoring.