Immunity, vaccination and rainbow trout family susceptibility to flavobacterium psychrophilum

由嗜冷黄杆菌（Flavobacteriumpsychrophilum）引起的细菌性冷水病（Bacterialcoldwaterdisease，BCWD）对加拿大淡水鲑鱼养殖业造成了严重的危害。随着一些鳟鱼品系的死亡率接近20-30%，以及相关的大量抗生素使用，疾病的成本是巨大的，抗生素耐药性的风险是严重的。尽管有明显的需求，但没有商业疫苗可用。此外，育种计划目前正在进行中，以改善虹鳟鱼的生长，但通过家庭选择计划取得的任何收益都不能被BCWD造成的死亡率增加或其他成本所抵消。由于这些原因，需要开发有效的管理工具，以减少这种疾病的有害影响。因此，该项目有三个主要目标：1。评价冷诱导蛋白作为疫苗候选物的价值; 2.鉴定虹鳟鱼BCWD抗性和易感家系，可用于了解虹鳟鱼抗病的免疫学基础;以更好地表征由疫苗接种以及自然和实验感染刺激的体液和细胞介导的免疫应答。该项目的结果应有助于提供预防BCWD的有效工具，并为四名博士候选人和几名暑期学生提供高质量的培训。

Bacterial coldwater disease (BCWD), caused byFlavobacterium psychrophilum, has a marked deleterious impact on the freshwater salmonid aquaculture in Canada. With mortalityof some trout strains approaching 20-30% and associated heavyantimicrobial use, the costs of the disease are substantial and the risk of antimicrobial resistance is serious. In spite of the obvious need, there are no commercial vaccines available. Further, breeding programs are currentlyunderwayto improve the growth of rainbow trout, but it is imperative that anygains made via a familyselection program aren't negated byincreased mortalityor other costs due to BCWD. For these reasons, development of effective management tools to reduce the deleterious impact of this disease are needed. This project therefore has three major goals: 1. to evaluate cold-induced proteins as vaccinecandidates, 2. to identifyBCWD resistant and susceptible families of rainbow trout that can be used to understand the immunological basis for disease resistance and 3. to better characterize the humoral and cell-mediated immune responses stimulated byvaccination and bynatural and experimental infection. The results of this project should help to deliver effective tools for the prevention of BCWD and provide high qualitytraining for four doctoral candidates and several summer students.