Preparative ultracentrifuge for the study of ribonucleoprotein complexes, their structure, function, and rational engineering.

莱斯布里奇大学迫切需要一种制备型超离心机来支持几个研究项目。这些项目关注的是细胞机制的结构和功能动力学的机制原理，这些机制负责将遗传信息翻译成功能性蛋白质，这一过程被称为翻译。翻译发生在核糖体上，核糖体是细胞纳米机器中最丰富的RNA和蛋白质之一。这些大分子机器以复杂和精确定时的方式与许多其他蛋白质和rna相互作用，促进对每个活细胞至关重要的快速准确的蛋白质合成。详细了解大分子组装过程中固有的动态特性和机制对于合理的药物设计和生物分子工程等各种应用至关重要。核糖体是一个特别相关和有趣的模型系统，是蛋白质合成的场所，也是许多抗生素和合理药物设计项目的目标。支持的研究项目将有助于理解和确定蛋白质- rna纳米机器及其自组装特性的设计原理。进一步阐明RNA修饰和修饰酶在含RNA和蛋白质的细胞机器的组装和功能机制中所起的作用，为生物分子纳米机器的合理设计和工程化提供关键信息。所要求的仪器将用于核糖体、核糖体亚基、核糖体前体以及确定的核糖体复合物和修饰的核糖体的分析和制备纯化。该仪器将取代现有的旧系统，该系统是莱斯布里奇大学唯一的制备型超离心机，今年早些时候发生了故障。所要求的超离心机将与现有的超离心转子套件兼容，使其成为最具成本效益的解决方案。

A preparative ultracentrifuge is urgently requested to support several research projects at the University of Lethbridge. These projects focus on the mechanistic principles underlying the structural and functional dynamics of the cellular machinery that are responsible for the translation of genetic information into functional proteins, a process called translation. Translation takes place at the ribosome, one of the most abundant RNA and protein containing cellular nano-machines. These macromolecular machines interact in a complex and precisely timed fashion with numerous additional proteins and RNAs, facilitating the fast and accurate protein synthesis crucial for every living cell. A detailed understanding of the dynamic properties and mechanisms inherent to processes occurring in macromolecular assemblies is crucial for a wide variety of applications such as rational drug design and bio-molecular engineering. The ribosome is a particularly relevant and interesting model system, being the site of protein synthesis and the target for numerous antibiotics and rational drug design projects. The supported research programs will strongly contribute to understanding and identifying the design principles underlying protein-RNA nano-machines and their self-assembly properties. Furthermore it will shed light on the role that RNA modifications and modifying enzymes play for the assembly and functional mechanisms of such RNA and protein containing cellular machines, providing us with key information required for the rational design and engineering of bio-molecular nano-machines. The requested instrument will be used for the analysis and preparative purification of ribosome, ribosomal subunits, ribosomal precursors, as well as defined ribosomal complexes and modified ribosomes. The requested instrument will replace an old existing system which is the only preparative ultracentrifuge at the University of Lethbridge and which failed earlier this year. The requested Ultracentrifuge will be compatible with the existing suite of ultracentrifugation rotors, making it the most cost-effective solution.