Functional analysis of GRASP in protein transport and Golgi architechture in the malaria parasite Plasmodium falciparum

所有已知的真核细胞都在自己体内形成了专门的隔室，以提供指定的反应室，以控制它们的新陈代谢。这些细胞通过进化出一个复杂的内膜系统来应对这一挑战。这种重新设计伴随着额外的费用：他们必须确保有一个系统在个体和亚细胞部门之间进行沟通。在这个系统中，高尔基仪器是操作的中心。尽管这种细胞器的极性及其顺式和反式面是普遍保守的，但在整个真核系统发育树中高尔基体的形态变化很大。在这个研究项目中，我们将探索疟疾寄生虫的高尔基体。这种对人类具有毁灭性影响的细胞内病原体开发了一种独特的运输系统来征服其生态位：人类肝细胞和红细胞。我们将利用寄生虫的“简单”高尔基形态及其蛋白质运输的专门机制来解决一些关于细胞器生物学和蛋白质运输的基本问题。为此，我们将重点研究GRAP的功能分析--一种与高尔基体结构和蛋白质运输有关的蛋白质。我们不仅将深入探讨细胞生物学的一些基本问题，还将启动翻译应用程序，将蛋白质运输作为治疗靶点。

All known eukaryotic cells developed specialized compartments within themselves to provide designated reaction chambers in order to control their metabolism. These cells mastered this challenge by evolving a complex endomembrane system. This re-design came with an additional expense: They had to make sure that there is a system in place that is mediating the communication between the individual, subcellular divisions. Within this system, the Golgi apparatus is the operational centerpiece. Although the polarity of this organelle with its cis and trans face is universally conserved, the morphology of the Golgi varies greatly throughout the eukaryotic phylogenetic tree. In this research program we will explore the Golgi of the malaria parasite. This intracellular pathogen with a devastating impact on mankind developed a unique transport system to conquer its ecological niche: the human hepatocytes and erythrocytes. We will exploit the "simple" Golgi morphology of the parasite and its specialized mechanism of protein transport to address some fundamental questions about organelle biology and protein transport. To do so we will focus on the functional analysis of GRASP - a protein that it is involved Golgi architecture and protein trafficking. We will not only probe deep into some elementary questions of cell biology but also initiate translational applications to exploit protein trafficking as a therapeutical target.