Analysis of genetic targeted mutations to improve drought tolerance in cereals

发展耐旱性是世界范围内保持作物产量的主要障碍。改善这种复杂性状的方法包括直接遗传修饰和育种或选择赋予所需特征的遗传突变。为了克服遗传工程的局限性和挑战，将利用反向遗传学（非转基因）策略来选择具有所需表型属性的植物，所述反向遗传学策略将传统诱变与单核苷酸变化的成本效益发现相结合。在这项提案中，我们将使用水稻作为模式谷类作物来分析和研究这些突变提高水分利用效率的潜力，并选择那些成功赋予非生物胁迫耐受性的等位基因，用于未来在其他作物（包括小麦和玉米）中的验证。农艺性状的发展是加拿大农业的一个关键优先事项。该项目将为评估遗传学奠定基础。 技术的商业潜力。成功的结果将使该公司能够继续开发和销售加拿大农业感兴趣的新品种。

Developing drought tolerance is a major obstacle in maintaining crop yields world-wide. Approaches to improve this complex trait include direct genetic modification and breeding or selection of genetic mutations conferring the desired characteristics. To overcome the limitations and challenges of genetic engineering, a reverse genetics (non-transgenic) strategy that combines traditional mutagenesis with cost effective discovery of single-nucleotide changes will be exploited to select plants with the required phenotypic attributes. In this proposal, we will use rice as a model cereal crop to analyse and investigate the potential of such mutations to improve water use efficiency and those alleles that successfully confer abiotic stress tolerance will be chosen for future validation in other crops of interest including wheat and corn. Development of agronomic traits is a key priority for the Canadian farming industry. This project will set the stage for assessing the genetic technology for commercial potential. Successful outcomes will allow the company to move forward with the development and marketing of new varieties of interest for Canadian agriculture.