G protein-coupled receptor interactome: Discovery and study of novel neighboring and interacting partners

本研究计划重点研究G蛋白偶联受体(GPCRs)的相互作用组。其目的是确定和调查新的邻近和互动伙伴在典型GPCRs的贩运和下游信号级联中的作用。为此，我们建议使用大肠杆菌生物素蛋白连接酶作为一种依赖于邻近的鉴定策略。这种创新的大规模筛选方法应该会导致发现新的合作伙伴，并在他们的“原始”蜂窝环境中发现GPCR的信号级联。

This research program focusses on studying the interactome of G protein-coupled receptors (GPCRs). The aim is to identify and investigate the roles of novel neighboring and interacting partners involved in the trafficking and the downstream signaling cascades of prototypical GPCRs. To this end, we are proposing to use the Escherichia coli biotin protein ligase as a proximity-dependent identification strategy. This innovative, large scale screening approach should lead to the discovery of novel partners and signaling cascades of GPCRs in their “native” cellular environment.