Confocal microscope for muscle health research

显微镜是生物和生命科学中科学研究的基本工具。 在约克大学，我们有大量的研究人员在生物系，运动学和健康科学学院，肌肉健康研究中心（MHRC），需要使用荧光共聚焦显微镜。 我们目前有一种荧光显微镜，可用于观察固定细胞中的细胞结构。 在这项申请中，我们正在申请资金来升级这个模型，以便对细胞的各个层进行活细胞成像，从而对细胞器（如线粒体、细胞核和溶酶体）进行更准确和详细的分析。 这是一种更强大的方法，用于研究不同生理条件下的细胞生物学（例如，肌肉收缩、药物治疗）。 作为MHRC的主任，我代表上述3个研究单位的许多（37）教职员工提交此申请，他们从事可能使用此设备的细胞生物学研究。 这些教师中有几个是经常使用它的主要用户。这台设备将增加我们进行这种基本细胞分析的能力，并消除目前前往另一座大楼使用约克大学校园内另一台功能仪器的用户的堵塞。

Microscopy is a fundamental tool for scientific investigation in the biological and life sciences. At York University we have a large number of researchers in the Department of Biology, the School of Kinesiology and Health Science, and the Muscle Health Research Centre (MHRC) that require the use of fluorescence confocal microscopy. We currently have a fluorescent microscope which is useful for visualizing cellular structures in fixed cells. In this application we are requesting funds to upgrade this model to allow for live cell imaging of various layers of the cell to allow for a more accurate and detailed analysis of cellular organelles, like mitochondria, nuclei and lysosomes. This is a much more powerful approach for the study of cell biology under different physiological conditions (eg. muscle contraction, drug treatment). As the Director of the MHRC, I am submitting this application on behalf of numerous (37) faculty members in these 3 research units above who do cell biology research that potentially could make use of this equipment. Several of these faculty members are major users who employ it regularly. This equipment will increase our capacity for this fundamental cell analysis and dissipate the logjam of users who currently travel to another building to use the one other functional instrument on the York University campus.