Epifluorescence microscopy workstation

细胞成像是细胞生物学研究的重要工具，也是生物化学、生理学和分子生物学等其他学科研究的重要工具。卡尔加里大学生物科学系的许多实验室依靠荧光显微镜来满足他们的研究需要。这项RTI赠款提案要求资金购买一台多用途、多用户的荧光显微镜工作站，以取代一台已有18年历史的故障工作站。这个失败的系统已经被生物科学系18个研究实验室以及理学院其他系的100多名HQP广泛和免费地使用和使用。这一用途有助于发表超过45篇同行评议的研究论文。将配置一个替换的显微镜工作站，以便继续广泛和免费地向系内所有成员和系内其他系成员提供。拟议的显微镜工作站的主要用户进行的研究是多学科的，并使用多细胞和单细胞系统，包括植物、锥虫、分裂和发芽酵母。研究领域包括转录后基因调控、油砂毒素生物传感和修复、花粉-雌蕊相互作用、萜类生物合成、脂类代谢和信号转导。不太频繁的使用者还研究细菌生物膜和动物模型细胞类型。一个多用户、多学科的荧光显微镜工作站对于我们系广泛的细胞生物学需求是必不可少的。我们老化的当前工作站不再提供我们所需的可靠性、容量、自动化或质量，迫切需要更换。替换系统将安装在位于生物科学大楼三楼的专用成像实验室中。

Cellular imaging is an important tool for cell biology research, as well as research from other disciplines, including biochemistry, physiology and molecular biology. Numerous laboratories in the Department of Biological Sciences at the University of Calgary depend on epifluorescence microscopy to meet their research needs. This RTI grant proposal requests funds to purchase a multipurpose, multiuser epifluorescence microscope workstation that will replace a failing 18-year old workstation. This failing system has been made broadly and freely available and used by over 100 HQP from 18 research labs in the Biological Sciences department, as well as other departments in the Faculty of Science. This use has contributed to the publication of over 45 peer reviewed research papers. A replacement microscope workstation will be configured so that it will continue to be broadly and freely available to all members of the department and other departments within the faculty. Research conducted by the major users of the proposed microscope workstation is multidisciplinary, and uses multicellular and single-celled systems that include plants, a trypanosome, and fission and budding yeast. Research areas include post-transcriptional gene regulation, oil sands toxin biosensing and remediation, pollen-pistil interactions, terpenoid biosynthesis, lipid metabolism and signal transduction. Less frequent users also study bacterial biofilms and animal model cell types. A multi-user, multidisciplinary epifluorescence microscopy workstation is essential for the broad cell biology needs of our department. Our ageing current workstation no longer provides the reliability, capacity, automation or quality that we require, and desperately needs replacement. The replacement system will be housed in a dedicated imaging laboratory located on the third floor of the Biological Sciences building.