QTRAP facility for trace metabolite detection and identification

目前迫切需要资金来替换渥太华大学自然和合成环境毒素分析核心设施（LANSET）中用于检测痕量代谢物和等压分子分化的3200 QTRAP设备（现已停用）。我们的研究人员经常需要在复杂的生物或环境样本中找到“大海捞针”，这些样本可能包括土壤、水或鱼中的毒素，植物中的植物化学物质，动物组织中的天然产物或合成药物。在过去的十年中，QTRAP设施对于我们的教师和许多合作机构进行具有挑战性的博士研究至关重要。在未来，该设施将对我们资助的项目至关重要，如湖泊藻类毒素的生态毒理学研究，淡水系统气候变化的生物标志物，水产养殖中不良植物雌激素的影响，以及加拿大和热带植物的天然产物研究，它们的抗焦虑和代谢生物活性，以及与药物和毒素相互作用的药代动力学风险。提出的4000 QTRAP技术结合了任何检测器的最高选择性方法之一，具有卓越的灵敏度，价格实惠，具有三重四极杆和离子阱质谱平台的全部功能。QTRAP设施将允许当前的研究生、pdf和研究助理在国际竞争水平上完成他们的研究。此外，该设施为外部合作研究人员和私营部门提供必要的支持，包括我们自己的（Arnason）分拆公司以及多家加拿大和国际公司。

Funding is urgently required to replace an essential and heavily used 3200 QTRAP facility (now defunct) for detection of trace metabolites and differentiation of isobaric molecules at the U of Ottawa Core Facility for the Analysis of Natural and Synthetic Environmental Toxins (LANSET). Our researchers frequently need to find the “needle in the haystack” in complex biological or environmental samples, which may range from a toxin in soils, water or fish, to a phytochemical in plants, to a natural product or synthetic drug in animal tissues. In the past decade, the QTRAP facility has been essential to undertake challenging PhD research in our faculty and with many collaborating institutions. In the future the facility will be essential for for our funded programs such as ecotoxicology research on algal toxins in lakes, biomarkers of climate change in freshwater systems, and undesirable phytoestrogen effects in aquaculture, as well as natural product research on Canadian and tropical plants, their anxiolyic and metabolic bioactivities, and risk of pharmacokinetic interactions with drugs and toxins. The proposed 4000 QTRAP technology combines one of the highest selectivity methods of any detector with remarkable sensitivity at an affordable price with the full functionality of both triple quadrupole and ion-trap MS platforms. The QTRAP facility will allow current graduate students, PDFs and research associates to complete their research at an internationally competitive level. In addition, the facility provides essential support to collaborating external researchers and the private sector, including our own (Arnason) spin off company and multiple Canadian and international companies.