Cannabis plant growth promotion using PGPR

许多有益的细菌生活在植物的根际(即靠近根部的土壤)，可以促进植物的生长。这些细菌被称为植物生长促进根际细菌(PGPR)，可能属于不同的分类群，包括假单胞菌。和芽孢杆菌属。假单胞菌的部分菌株。和芽孢杆菌属。可通过多种机制直接和/或间接刺激植物生长。和芽孢杆菌属。它们是替代和/或减少化肥使用的理想候选者，因为它们很容易培养和接种，而且它们的生产成本是替代化肥应用的合理选择。虽然大量的假单胞菌属的菌株。和芽孢杆菌已被鉴定为在不同系统中潜在的PGPR，但假单胞菌/芽孢杆菌组合对植物生长的协同影响还没有得到广泛的研究。多年来，我的研究团队已经分离并鉴定了大量假单胞菌菌株。因为它们具有促进植物生长的能力。与此同时，生物技术公司Ulysse已经在分离和鉴定芽孢杆菌方面开发了大量的专业知识。用于促进植物生长的菌株。因此，我们的专业知识是互补的，可以促进微生物联盟的发展，展示出出色的协同植物生长促进活性。生物技术公司Ulysse正在寻求帮助，成为首批能够将专门为大麻行业量身定做的环境友好型微生物肥料商业化的加拿大公司之一。因此，在本项目中，收集了我们实验室分离的60株不同的假单胞菌，并与两株芽孢杆菌进行了联合测试。菌株(由生物技术公司Ulysse拥有)是因为它们有能力促进大麻的生长和生产力。更具体地说，其目的是：1)鉴定最适的假单胞菌/芽孢杆菌。2)为这些生物开发特定的DNA分子标记，从而能够使用qPCR分析来检测和量化它们在土壤和植物条件下的种群动态。

Many beneficial bacteria living in the rhizosphere of plants (i.e. the soil in close proximity to roots) canpromote plant growth. These organisms are known as plant growth promoting rhizobacteria (PGPR) and maybelong to different taxonomical groups, including Pseudomonas sp. and Bacillus sp. Some strains ofPseudomonas sp. and Bacillus sp. can stimulate plant growth directly and/or indirectly by various mechanisms.Pseudomonas sp. and Bacillus sp. are ideal candidates for replacing and/or reducing the use of chemicalfertilizers as they can be easily cultured and inoculated, and their production cost represents a reasonablealternative to chemical fertilizer applications. Although numerous strains of Pseudomonas sp. and Bacillus sp.have been identified as potential PGPR in different systems, combinations of Pseudomonas sp./Bacillus sp.strains that can synergistically impact on plant growth have not been widely studied. Over the years, myresearch team has isolated and characterized numerous strains of Pseudomonas sp. for their plant growthpromoting capacities. In parallel, Biotechnology Ulysse has developed a large expertise in isolating andcharacterizing Bacillus sp. strains for plant growth promotion. Therefore, our expertise is complimentary andcan lead to the development of microbial consortia that display superior synergistic plant growth promotingactivity. Biotechnologies Ulysse is seeking help to be among the first Canadian companies able tocommercialize environmentally-friendly microbial-based fertilizers specifically tailored to the Cannabisindustry. Therefore, in this project, a collection of 60 different Pseudomonas sp strains isolated in ourlaboratory will be tested in combination with two Bacillus sp. strains (owned by Biotechnologies Ulysse) fortheir capacity at promoting Cannabis growth and productivity. More specifically, the aims are to: 1) Identify themost optimal Pseudomonas sp./Bacillus sp. combination capable of promoting Cannabis plant growth; and 2)Develop specific DNA molecular markers for these organisms allowing to detect and quantify their populationdynamics under soil and plant conditions using qPCR assays.