Optical Coherence Tomography, Molecular Guided Imaging, and Surgical Navigation for targeted and controlled Laser Ablation

PI Yang的这一提案继续致力于为神经外科手术室（OR）及其他领域开发图像引导技术。*目标 * 我们的广泛目标包括：* 1）开发用于组织检测和描绘的新型成像方法 * 2）融合用于显示和手术导航的成像方法 * 3）用于治疗靶向的图像引导和融合 * 4）用于监测治疗效果的成像方法 * 具体目标是：* 1）继续研究结构和血管散斑方差和多普勒光学相干层析成象术SV/D-OCT）方法用于检测和描绘脉管系统，采用这些方法进行功能成像 * 2）继续发展OCT的弹性成像方法以描绘各种组织类型，包括脑组织结构和健康与患病组织 * 3）分子荧光技术的持续发展，重点是采用定量，深度分辨，* 4）OCT和荧光数据与当前MVIGS系统的融合 * 5）优化信息工作流程以防止认知过载 * 6）将导航信息转换为机器人-安装的消融激光器，用于通过OCT控制对结构进行靶向和受控消融 * 技术焦点、方法、迄今为止的工作 * BBL之前建立的核心技术将继续开发，具体如下：* 1）OCT：一种基于红外线的干涉测量方法，横向分辨率为10微米，深度成像可达3毫米。我们继续开发用于结构和血管成像（SV/D-OCT）以及OCE的方法，并将继续将这些方法应用于外科手术。2)分子引导荧光成像：这种方法使用特定的分子荧光团来实现目标成像。我们已经开发了一个时间控制的生物光子手术室，允许在不中断工作流程的情况下进行实时成像，并正在努力建立定量方法。3)机器视觉图像引导手术（MVIGS）：我们开发了一种导航系统，将结构光与术前成像相结合，用于工具定位。我们的目标是继续研究开发方法，将其他成像模式与我们当前的导航系统集成，以优化工作流程。* 4)引导激光消融：我们已经开发出OCT控制的激光消融方法，使用尺寸与笔记本电脑相似的光纤激光器。我们目前正在努力表征这些系统，并使用MVIGS与机器人进行宏观指导，OCT和荧光进行微观指导。影响 * 上述研究对于将这些新技术转化为OR具有特别重要的意义。我们的目标是将这些技术引入手术室，并考虑到工作流程，并将其应用范围从神经外科手术室扩展到其他外科和医学领域，并有机会产生知识产权和创造就业机会。

This proposal from PI Yang continues work in the development of image-guided techniques for the neurosurgical operating room (OR) and beyond.***Objectives***Our broad goals include:***1) Development of novel imaging methods for tissue detection and delineation***2) Fusion of imaging methods for display and surgical navigation***3) Image-guidance and fusion for therapeutic targeting***4) Imaging methods for monitoring of therapeutic effects***Specific aims are:***1) Continued work on structural and vascular (Speckle Variance and Doppler Optical Coherence Tomography; SV/D-OCT) methods for the detection and delineation of vasculature, with adoption of these methods for functional imaging***2) Continued development of elastography methods for OCT for the delineation of various tissue types, including brain tissue structures and healthy vs. diseased tissues***3) Continued development of molecular fluorescence techniques, with focus on the adoption of quantitative, depth resolved, and 3D methods***4) Fusion of data for OCT and fluorescence with current MVIGS system***5) Optimization of information workflow to prevent cognitive overload***6) Translation of navigation information to robot-mounted ablation lasers for targeted and controlled ablation of structures with OCT control***Technological foci, methods, work to date***Core technologies previously established in by the BBL will continue to be developed, specifically:***1) OCT: An infrared based interferometric method with 10 micron lateral resolution, and depth imaging of up to 3mm. We have continued to develop methods for structural and vascular imaging (SV/D-OCT), as well as OCE, and will continue to translate these to a surgical setting.***2) Molecular guided fluorescence imaging: This method uses specific molecular fluorophores to allow imaging of targets. We have developed a temporally controlled biophotonics operating room allowing for real-time imaging without disruption of workflow, and are working to establish quantitative methods.***3) Machine Vision Image Guided Surgery (MVIGS): We have developed a navigation system that combines structured light with presurgical imaging for tool localization. We aim to continue to look at developing methods for the integration of other imaging modalities with our current navigation system for optimized workflow.***4) Guided Laser Ablation: We have developed methods for OCT-controlled laser ablation using fiber lasers in a size-envelope similar to that of a laptop. We are currently working to characterize these systems, and to use MVIGS with robotics for macroscopic guidance, and OCT and fluorescence for micro-guidance.***Impact***The above research is of particular importance to allow for the translation of these novel technologies into the OR. We aim to bring these technologies into the OR with workflow considerations in mind, and to expand their use beyond the neurosurgical operating room to other areas of surgery and medicine, with opportunity for IP generation and job creation.**