Histology core equipment

此应用程序是为了更新我们的组织学核心设备（处理，包埋和切片机站）。现有设备已使用10年以上，经过多次修理，已经过时。该设备对用户或环境也不安全，因为该空间没有足够的通风来容纳应放置在通风柜内的处理器。这造成了组织处理和学生项目的延迟，并增加了我们的发现赠款无法支付的额外成本。我们想购买一个独立的碳过滤器封闭系统，该系统将保护用户和环境，以处理组织沿着包埋站，因为我们现有的系统不再冷却样本，我们必须有一个额外的冷板来阻挡组织。我们还想把手动切片机升级为半自动切片机。升级不仅更加用户友好，而且使我们能够进行更一致的组织切片，并且具有保护用户的额外安全功能。* Richard博士的发现资助的一个重要的新方面是了解Wistar大鼠肥胖模型中肠道形态、肠道相关免疫系统和外周或全身免疫系统之间的联系。这需要在肠道的不同部分进行免疫组织化学。雅各布斯博士的发现基金致力于了解饮食胆碱和相关营养素对哺乳动物生理学的作用。在这些程序中，组织学分析的重要性不能被夸大。我们目前使用的设备在过去一年中已经维修过多次，维修部件并不总是可用，延迟了方案优化和HQP培训。这证明有必要和迫切需要现在就更换它，以防止进一步拖延。许多其他合作者，教授和他们的学生在部门使用组织学核心设备。由于组织学核心有多个用户，该部每天提供一名技术员操作和维护该设备，使其更易于使用。她还培训学生和其他HQP如何使用这些设备。** 我们开发了一个计划，学员接触研究的各个方面，从基础科学到转化应用，即“从试管到动物模型到人类”，使用各种实验方法，包括生物化学，细胞生物学，分子生物学和体内技术。 这使得我们的HQP在他们的专业“工具包”中有许多“工具”，这将提高他们的就业能力。学习如何使用组织学设备以及如何分析结果的学员获得了宝贵的技能，这些技能在许多不同的职业（工业，医疗保健，学术界等）中都是一笔财富。每年，我们培训了许多HQP，从研究助理，新的实验室技术人员，研究生以及来自不同部门的本科生。 **

This application is to update our histology core equipment (processing, embedding and microtome stations). The existing equipment is over 10 years old, have been repaired numerous times and is obsolete. The equipment is also not safe for the users or the environment as the space does not have sufficient ventilation to accommodate the processor which should be placed within a fumehood. This creates delays in tissue processing and students' project and adds additional cost that our discovery grants cannot cover. We would like to buy a free standing carbon filter enclosed system that will protect both the users and the environment for processing tissues along with the embedding station since the one we have does not cool samples anymore and we have to have an additional cold plate for blocking tissue. We also want to update the manual microtome to a semi-automated. The upgrade is not only more user friendly but would allow us to have more consistent tissue sectioning and it has additional safety features that protect the users.******An important novel aspect of Dr. Richard's Discovery grant is to understand the connection between the gut morphology, the gut-associated immune system and the peripheral or systemic immune system in the Wistar rat model of obesity. This requires to do immunohistochemistry on different sections of the gut. Dr. Jacobs' Discovery grant strives to understand the role of dietary choline, and related nutrients, on mammalian physiology. The importance of histological analysis cannot be overstated within these programs. The current equipment we are using has been repaired numerous times in the last year and parts for the repairs are not always available delaying protocol optimization and the training of HQPs. This justifies the need and urgency of replacing it now to prevent further delay. Many other collaborators, professors and their students in the Department use the histology core equipment. Since the histology core has multiple users, the Department provides a technician to operate and maintain the equipment on a daily basis which makes it more affordable to use. She also trains students and other HQPs on how to use the equipment. ******We have developed a program where trainees are exposed to all aspects of research, from basic science to translational applications i.e. “from test-tube to animal models to humans” using a variety of experimental approaches that include biochemical, cell biological, molecular biological, and in vivo techniques. This allows our HQP to have many “tools' in their professional “tool kit” that will improve their employability. Trainees who learn how to use histology equipment and how to analyze the results gain valuable skills that are an asset in many different careers (industry, healthcare, academia etc.). Each year, we train a number of HQPs that span from research associate, new lab technicians, graduate students as well as undergraduate students from different Department. **