System for Ultra-high Speed Imaging of Sonochemical Phenomena in Biological Cells and Transmitting Medium during Ultra Fast Phase Transformation

尽管申请人的光学生物微系统实验室拥有各种最先进的研究设备，但迫切需要与HIFU声源同步的超高速成像设备，适合生物和工程应用。生物应用包括在各种内化环境（如纳米颗粒和外泌体）下研究声化学与细胞（包括神经元和其他癌细胞）的相互作用。这样的设施还将加强正在进行的研究，以开发工程材料的超快速相变（UFPT）领域的尖端技术，以及细胞中的声纳相互作用。支持的研究项目包括但不限于：1)upt微粒子合成，2)选择性纳米粒子合成，3)聚焦超声神经调节的声化学研究。利用光子聚焦超声对细胞的声化学研究使我们的团队能够对上述项目进行研究。超高速FASTCAM SA-Z通过其高度光敏图像传感器（单色ISO 50,000）提供帧率高达21,000 fps的百万像素图像分辨率，具有12位动态范围，提供终极成像性能。FASTCAM SA-Z在降低图像分辨率的情况下提供超过200万帧/秒的帧速率，快门速度短至159纳秒。获得该设备，不仅可以促进声相互作用下生物和材料现象的实时成像，而且可以保持前沿研究成果，加强培养目标，提高论文质量，缩短毕业时间。我们的小组目前有30多名研究生和5名博士后在研究项目中工作，这些项目将从这个设施中受益。将使用该设施的合作者来自古德曼癌症研究中心和麦吉尔大学头颈外科，康科迪亚大学生物和工程系以及蒙克顿的大西洋癌症研究所。除了进行尖端研究外，该设备还使团队能够验证他们的建模和模拟。我们相信，这些实验室围绕该设备进行多学科研究的合作，将带来突破性的认识和实用的产品，这将为工业带来巨大的市场。三个研究机构围绕该设备的合作包括声学、材料开发、细胞生物学、头颈癌治疗、神经元生物学和外泌体相互作用等领域的各种应用。提出的研究计划将为声-生物相互作用和新材料的开发开辟新的方法，用于许多应用。该系统位于光学生物微系统实验室，将向现场和加拿大各地的其他机构合作开放，包括麦吉尔大学、UdeM、ETS和École Polytechnique.*****

Even though the Optical Bio Microsystems Laboratory of the applicant houses various state-of-the-art research facilities, there is a dire need for Ultra-High Speed Imaging Facility synchronized with HIFU acoustic source, suitable for both biological and engineering applications. Biological applications include study of sonochemistry interactions with cells including neurons and other cancer cells under various internalized environments such as nano particles and exosomes. Such a facility would also enhance ongoing research on developing cutting edge technologies in the area of Ultra Fast Phase Transformation (UFPT) of engineering materials as wells as sono interactions in cells. The supported research programs include but not limited to: 1) Micro particle synthesis in UFPT, 2) Selective nano particle synthesis, 3) Sonochemical Investigation of Neuromodulation using Focused Ultrasound. The Sonochemical Investigation of cells using Focused Ultrasound from Photron enables our groups to conduct research on the mentioned programs. The ultra-high speed FASTCAM SA-Z provides megapixel image resolution at frame rates up to 21,000 fps from its highly light sensitive image sensor (monochrome ISO 50,000) with 12bit dynamic range delivering the ultimate imaging performance. The FASTCAM SA-Z provides frame rates greater than 2 million fps at reduced image resolution and shutter speeds as short as 159 nanoseconds. Acquiring this equipment would not only facilitate the real time imaging of biological and material phenomena under acoustic interactions but would also sustain leading-edge research success, strengthen training goals, enhance quality of dissertations, and shorten graduation time. Our groups currently have more than 30 graduate students and 5 postdocs working on the research programs that will benefit from this facility. The collaborators that will use the facility are from Goodman Cancer Research Center and Head and Neck Surgery Department of McGill University, Biological and Engineering Departments of Concordia and Atlantic Cancer Research Institute at Moncton. In addition to conducting cutting edge research, the equipment enables the team to validate their modeling and simulations also. We believe that the collaboration of these labs in multidisciplinary research around this equipment will lead to break through understandings and practical products that will have huge market for industries. The collaborations between three research institutes around this equipment include diverse applications in acoustics, materials development, cellular biology, head and neck cancer treatment, neuronal biology and exosomal interactions. The proposed research programs will open up new methodologies for acousto-bio interactions and new material development for many applications. Housed in Optical-bio Microsystems Lab, the system will be open to on-site and other inter-institutional collaborations across Canada including McGill, UdeM, ETS and École Polytechnique.*****