Flow Cytometer for Muscle Health Research

******流式细胞术是一种允许研究人员使用荧光标记技术检查单个细胞或源自细胞的细胞器的生化特性的方法。它依赖于激光的使用，激光产生不同的波长，可以用来激发和探测细胞或细胞器内的分子。在这份申请中，我要求获得资金，在整个约克校区购买第三个这样的设备系统，并取代我的过时的系统，这个系统在过去为我们服务得很好，但现在维护成本高于使用成本，而且现在已经不能运行了。我的学员和同事将继续使用流式细胞术分析未成熟（成肌细胞）和成熟状态（肌管）肌肉细胞的生化特性，以及健康对照者和线粒体肌病患者皮肤成纤维细胞的特征。我们还使用流式细胞术分析分离的线粒体和线粒体磷脂的功能(例如。从健康和锻炼的动物模型中提取心磷脂，以支持我们的NSERC发现资助目标。获得这种新设备对于我们在实现NSERC DG研究目标方面取得进展是绝对必要的。延迟收购将对我们的进展产生重大影响，因为我们正在等待替代者。此外，该设备将对HQP的培训产生重大影响。我的实验室对硕士、博士和博士后的需求很大，目前每年有12名学员。流式细胞仪系统将使我能够培训这些HQP，使他们掌握市场上的、复杂的和最新的技术，这些技术将很好地为他们在学术和其他研究和分析环境中服务。此外，作为肌肉健康研究中心（MHRC）的主任，我也提交了这份申请，以帮助支持生物学系、运动与健康科学学院和肌肉健康研究中心的30多名其他教职员工，目标是提高我们目前非常有限的细胞分析能力，使用这一重要的方法在约克大学。**

******Flow Cytometry is a method which allows the researcher to examine the biochemical properties of individual cells, or organelles derived from cells, using fluorescent labeling techniques. It relies on the use of lasers which produce different wavelengths which can be used to excite and detect molecules within those cells or organelles. In this application, I am requesting funds to acquire only the third such equipment system on the entire York campus, and to replace my obsolete system which served us well in the past, but which now costs more to maintain than to use, and is now not operational. My trainees and colleagues will continue to use flow cytometry to analyze the biochemical properties of muscle cells in their immature (Myoblasts) and mature state (myotubes), as well as the characteristics of skin fibroblasts from healthy controls as well as mitochondrial myopathy patients. We also use flow cytometry to analyze the function of isolated mitochondria and mitochondrial phospholipids (eg. Cardiolipin) from healthy and exercised animal models, to support to support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we are waiting for a replacement. In addition, this equipment will have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 12 trainees on a yearly basis. The flow cytometry system will allow me to train these HQP in marketable, sophisticated and up-to-date technology, which will serve them well in academic and other research and analysis settings. In addition, as the Director of the Muscle Health Research Centre (MHRC), I am also submitting this application to help support the >30 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for cellular analyses using this important methodology at York University. **