Magnetic Tweezer Force Spectroscopy for Mechanotransduction Studies

所要开展的工作的性质：冠状动脉闭塞等心血管疾病是加拿大的主要死亡原因之一。对于严重冠状动脉阻塞的患者，经常使用导管为基础的干预措施，如冠状动脉成形术和支架置入术。然而，血管成形术和支架植入术受到平滑肌细胞不受控制的增殖的限制，平滑肌细胞最终会堵塞动脉。通过NSERC DG和DAS的持续资助，该研究项目将专注于开发新的使能技术，通过Jagged1 Notch配体与支架的功能偶联来解决与支架相关的挑战，目的是改善血管修复效果。该RTI资金申请是为研究计划购买急需的磁镊子力谱。******这项研究为什么重要，对谁重要，以及预期的结果：这项拟议的研究很重要，因为它将生物材料和生物学结合起来研究心脏病。拟议的研究将提供对支架性能尚未解决的挑战之一的基本理解。申请人期望开发具有共轭Jagged1的支架，以阻止不希望的平滑肌细胞增殖，从而导致再咬合。在此过程中，获得基本的材料诱导细胞生物学知识也是预期的。******对研究领域和加拿大的好处：拟议的研究利用一种生物材料（支架）将Jagged1呈现给细胞。拟议的研究将通过开辟医疗支架和基于生物材料的治疗技术领域的新市场，为加拿大未来人类健康和经济效益的应用带来重大技术发展

The nature of the work to be done: Cardiovascular disease such as coronary artery occlusion is one of the leading causes of death in Canada. For patients with severe coronary artery blockage, catheter-based interventions such as coronary angioplasty and stenting are frequently utilized. However, angioplasty and stenting are limited by the undesired and uncontrolled proliferation of smooth muscle cells that eventually reocclude the artery. Through the ongoing NSERC DG and DAS funding, the research program will focus on developing novel enabling technologies to address the challenges associated with stenting by functional conjugation of Jagged1 Notch ligand to stents with the aim of improving vascular reparative outcome. This RTI funding request is to purchase a critically needed magnetic tweezer force spectroscopy for the research program.******Why and to whom the research is important and the anticipated outcomes: The proposed research is important because it brings together biomaterials and biology to heart disease. The proposed research will provide fundamental understandings about one of the unsolved challenges in stent performance. The applicant anticipates to develop stents with conjugated Jagged1 that arrests the undesired smooth muscle cell proliferation that leads to reocclusion. In the process, gaining fundamental materials-induced cell biology knowledge is also anticipated. ******The benefits to the research field and to Canada: The proposed research utilizes one type of biomaterials (stents) to present Jagged1 to cells. The proposed research will result in significant technology development for applications in future human health and economic benefit to Canada by opening up new markets in the medical stent and biomaterials-based therapeutic technology sector.**