Development of integrated bioanalyte sensors for process monitoring in closed system bioreactors

干细胞培养的有效扩增和分化仍然是干细胞及其分化产物在再生医学中应用的瓶颈。为了支持细胞制造和研究，必须开发新技术来提高培养系统的性能。在这个项目中，我们建议通过开发用于培养物的集成实时监测和自动化生物过程控制的系统来克服这个瓶颈。为了实现这一目标，我们组建了一支独特的研究团队，他们拥有开发超灵敏多分析物芯片传感器、干细胞生物学、工程学和化学的专业知识。该团队将开发精确控制干细胞生产的技术。该项目的目标将为先进的再生医学疗法创造具有重要价值的个人技术，并将整合方法，为治疗细胞类型的分子水平控制提供更强大的解决方案。

Efficient expansion and differentiation of stem cell cultures remains a bottleneck in the use ofstem cells and their differentiated products in regenerative medicine. In order to support cellmanufacturing and research, novel technologies must be developed to increase theperformance of culture systems. In this project, we propose to overcome this bottleneck bydeveloping systems for integrated real-time monitoring of cultures and automated bioprocesscontrol. To achieve this goal, we have assembled a unique team of researchers with expertisein development of ultrasensitive multi-analyte chip-based sensors, stem cell biology,engineering, and chemistry. This team will develop technologies for precision control of stemcell production. The aims of this project will create individual technologies of significant valuefor advanced regenerative medicine therapies, and will also integrate methods that willprovide even more powerful solutions for molecular-level control of therapeutic cell types.