Upgrading high-resolution mass spectrometry system for advancing research in emerging environmental pollutants

我们需要两台外围仪器来升级高分辨率质谱（HRMS）系统。所要求的不含pfas的超高高效液相色谱（UHPLC）将建立UHPLC- hrms配置，而所要求的具有梯度加热功能的直接实时分析（DART）离子源将创建DART- hrms配置。升级后的HRMS系统将支持多个研究计划和项目，调查：(i)单氟烷基和多氟烷基物质（PFASs）的环境命运、运输、影响和补救；（ii）水生和城市环境中微塑料的环境命运。这些仪器将解决长期存在的高PFAS背景、大量积压的样品分析以及缺乏HQP培训机会的问题。这两种结构的互补使用将使我们能够有效地处理各种样品类型，并提取丰富的化学信息，否则是不可能的。第一种配置将能够对受PFAS污染的样品进行敏感、可靠和全面的表征，以跟踪破坏C-F键的新型生化反应中的氟，制定补救策略以保护饮用水供应，并确定食品和消费品中的PFAS。第二种配置将能够快速筛选样品，只需很少的预处理，并开发新的表征方法，用于高灵敏度和准确性的PFAS和塑料污染的批量监测。所要求的仪器将用于目前全球范围内的14个跨学科项目（600万美元），包括5个NSERC发现资助项目。来自加拿大、美国和英国公共和私营部门的至少14个合作组织也将从这些文书中受益。这些设备将在未来三年内用于在三个省不同学生群体的几个校区立即培训36名HQP。最先进的升级系统将大大提高HQP的培训和研究质量，以维持未来加拿大劳动力在环境和化学工程、应用微生物学和纳米材料领域的竞争力。

We are requesting two pieces of peripheral instruments to upgrade a high-resolution mass spectrometry (HRMS) system. The requested PFAS-free ultrahigh performance liquid chromatography (UHPLC) will establish the UHPLC-HRMS configuration, while the requested Direct Analysis in Real-Time (DART) ion source with gradient heating functionality will create the DART-HRMS configuration. The upgraded HRMS system will support multiple research programs and projects which investigate: (i) the environmental fate, transport, effect, and remediation of per- and poly-fluoroalkyl substances (PFASs) and (ii) the environmental fate of microplastics in aquatic and urban environments. The instruments will solve the long-standing issues of high PFAS background, the significant backlog of sample analysis, and the lack of opportunity for HQP training. The complementary use of the two configurations will enable us to efficiently deal with various sample types and extract rich chemical information that otherwise is not possible. The first configuration will enable the sensitive, reliable and comprehensive characterization of PFAS-contaminated samples for tracking fluorine in novel biochemical reactions that break C-F bonds, devising remediation strategies to protect drinking water supplies and identifying PFAS in food and consumer products. The second configuration will enable quick screening of samples with little pre-treatment and the development of new characterization methods for bulk monitoring of PFAS and plastic pollution with high sensitivity and accuracy. The instruments requested will be used in 14 current interdisciplinary projects (> $ 6 million) of global reach, including 5 NSERC Discovery Grants. At least 14 collaborating organizations from the public and private sectors in Canada, the US and UK will also benefit from the instruments. The equipment will be used to immediately train 36 HQP in the next 3 years at several campuses of diverse student populations across three provinces. The state-of-the-art upgraded system will substantially enhance HQP training and quality of research to sustain the competitiveness of the future Canadian workforce in the fields of environmental and chemical engineering, applied microbiology, and nanomaterials.