Prototyping and Deploying Air and Surface Virus Monitoring Point-of-Need Technologies to Improve Remediation Responses and Minimize Economic Losses of Viral Outbreaks

该项目是约克大学和第六波公司(SIXW)合作伙伴关系的扩展。它带来了新的合作伙伴，如Poppy Inc.、BluMetric Env。加拿大食品检验局(CFIA)和加拿大卫生部(HC)利用他们在环境生物传感器开发和验证方面的专业知识。目标：1)开发两种低成本的生物传感器，用于监测空气中和地表的敏感病毒；2)将生物传感器部署到终端用户实验室；3)评估合作伙伴的技术采用和经济效益。项目目标是与SIXW和Poppy合作开发便携式荧光测定仪(Obj.1)和电动(Obj.2)用于空气和表面病毒检测的生物传感器(目标1)通过以下活动：1)将生物传感器与便携式空气采样器、泵、电子和显微镜模块封装；2)对便携式生物传感器进行参数研究和优化；3)在实验室中确定其性能特性。OBJ。3是在现场部署用于在真实样本中检测病毒的生物传感器(目标2)。活动包括：1)将两个生物传感器转移到HC、CFIA、BME等，并对其进行优化，以处理真实的空气和表面病毒样本；2)培训合作伙伴人员使用生物传感器并共同执行验证研究。OBJ。4)了解生物传感器的采用障碍，以最大限度地提高生物传感器的经济和社会效益(目标3)，开展以下活动：1)确定和量化生物传感器的短期和长期经济和社会净效益；2)确定和评估采用的障碍。该项目使共同开发人员能够在了解用户对采用的障碍后将两种生物传感器推向市场，并帮助共同验证者实现快速环境监测，以便在病毒传播期间做出有效的补救行动和决定。将通过培训生物技术领域的6名本科生、2名理学硕士、2名博士生和2名博士后研究员，发展有关分子印迹聚合物及其对病毒亲和力的新知识。他们将传播专利和科学文章，同时申请未来的拨款，并获得/创造就业机会，将其技术商业化。

This project expands on a partnership between York U and Sixth Wave Inc. (SIXW). It brings in new partners like Poppy Inc., BluMetric Env. Inc. (BME), Canadian Food Inspection Agency (CFIA) and Health Canada (HC) to leverage their expertise in development and validation of environmental biosensors. Goals: 1) develop two low-cost biosensors for sensitive virus monitoring in air and on surfaces; 2) deploy the biosensors to end-user labs; and 3) assess technology adoption and economical benefits by partners. Project objectives are to partner with SIXW and Poppy to prototype a portable fluorometric (Obj. 1) and an electric (Obj. 2) biosensor for air and surface virus detection (Goal 1) by pursuing these Activities: 1) Package the biosensors with portable air sampler, pump, electric and microscopy modules; 2) Parametrically study and optimize the portable biosensors; 3) Determine their performance characteristics in-lab. Obj. 3 is to deploy the biosensors for virus detection in real samples, on-site (Goal 2). Activities include: 1) Transfer the two biosensors to HC, CFIA, BME, etc. and optimize them to work with real air- and surface-virus samples; 2) Train partner personnel to use the biosensors and co-perform validation studies. Obj. 4 is to understand barriers to adoption to maximize economic and societal benefits of the biosensors (Goal 3) by pursuing the following Activities: 1) Identify and quantify short- and long-term economic and societal net benefits of biosensors; 2) Identify and assess barriers to adoption.The project enables co-developers to bring two biosensors to the market after understanding user barriers to adoption, and help co-validators achieve rapid environmental monitoring to make effective remediation actions and decisions during virus spreads. New knowledge about molecularly imprinted polymers (MIPs) and their affinity to viruses will be developed by training 6 undergraduate, 2 Master of Science, and 2 doctoral students as well as 2 postdoctoral fellows, in the biotechnology sector. They will disseminate patents and scientific articles while applying for future grants and acquiring/creating jobs to commercialize their technologies.