Development of a Rational Basis for Scaling Bioreactors for Production of Proteins from Cloned Eukaryotic Genes and Viral Pesticides Using Insect Cell Cultures

昆虫细胞培养比哺乳动物细胞培养具有潜在的优势，可以从重组DNA中生产蛋白质。与使用幼虫生产病毒杀虫剂相比，它们也有优势。需要开发有效的大规模工艺策略来开发这些潜力。两个关键的障碍是对细胞生长和病毒繁殖机制如何响应培养条件变化的理解不足，以及无法将这些知识转化为易于扩展的生物反应器的设计和操作。建议进行综合研究以减少这些障碍。流体动力学和动力学模型也有待开发和验证。

Insect cell cultures offer potential advantages over mammalian cell cultures for production of proteins from recombinant DNA. They also offer advantages over the use of larvae for the production of viral insecticides. Efficient large scale process strategies to exploit these potentials need to be developed. Two key barriers are an inadequate understanding of how cellular growth and viral reproductive mechanisms respond to changes in culture conditions, and an inability to translate such knowledge into the design and operation of easily scalable bioreactors. An integrated research effort is proposed to reduce these barriers. Fluid dynamic and kinetic models are also to be developed and verified.