Design of Bioreactors for Insect Cell Cultures for the Production of Viral Pesticides and Foreign Gene Products

昆虫细胞组织培养可能被用于生产病毒杀虫剂，以及生产从重组DNA中获得的蛋白质，而这些蛋白质不能在细菌或酵母细胞中制造。目前还没有实用的大规模昆虫组织培养系统，如果有的话，可能会对上述材料的生产产生巨大的经济影响。这项提议寻求开发一种适合于昆虫细胞培养的新型生物反应器，该生物反应器还可以扩大规模，同时提供对微环境的高水平控制。拟议的工作真正具有创新性和新颖性，符合新奇研究快速奖的标准(见所附AD/ENG公告第85-25号)。私人投资促进机构被认为相当有能力进行拟议的研究，我强烈建议根据与建议一起提交的预算为这项建议提供资金。

Insect cell tissue cultures can potentially be used for the production of viral insecticides and for the production of proteins obtained from recombinant DNA that can not be made in bacterial or yeast cells. A practical large scale insect tissue culture system is not yet available and if it were, it could have immense economic implications for the production of the above materials. This proposal seeks to develop a novel bioreactor suitable for the cultivation of insect cells that is also amenable to scale-up while providing a high level of control over the microenvironment. The proposed work is truly innovative and novel and meets the criteria for Expedited Awards for Novel Research (see attached AD/ENG Bulletin No. 85-25). The PIs are considered to be quite capable of carrying out the proposed research and I strongly recommend that this proposal be funded in accordance with the budget submitted with the proposal.